Journal Article

Systematic Evaluation of Self-Adjuvanting Lipopeptide Nano-Vaccine Platforms for the Induction of Potent CD8 + T-cell Responses

Stephenson, Rachel J, Groves, Penny L, Aljohani, Salwa, Apte, Simon H, Toth, Istvan, Simerska, Pavla, Eskandari, Sharareh, Doolan, Denise L

2016

Overview

Systematically evaluate lipid core peptide vaccine delivery platforms to identify core features promoting strong CD8(+) T-cell responses. Three different self-adjuvanting lipid core peptide nanovaccines each comprising four copies of the dominant ovalbumin CD8(+) T-cell epitope and varying in the utilization of a polylysine or glucose core with 2-amino-hexadecanoic acid (C16) or 2-amino-dodecanoic acid (C12) lipids were synthesized. Vaccines were tested for ability to induce CD8(+) T-cell responses and inhibit tumor growth in vivo. The construct utilizing C12 lipids and polylysine core induced very robust effector T cells shown to have in vivo effector capability as demonstrated by in vivo cytotoxicity and ability to inhibit tumor growth as well as modulation of dendritic cell activation. The C12 polylysine platform was an effective configuration for induction of potent CD8(+) T-cell responses.

• Publisher: Future Medicine Ltd
• Subject: Animals / Antigens / Cancer Vaccines - administration & dosage / Cancer Vaccines - chemistry / CD8-Positive T-Lymphocytes - drug effects / CD8-Positive T-Lymphocytes - immunology / Dendritic cells / Lauric Acids - chemistry / Lipids / Lipopeptides - administration & dosage / Lipopeptides - chemistry / Lymphatic system / Lymphocytes / Mice / Mice, Inbred C57BL / Nanocapsules - chemistry / Nanocapsules - ultrastructure / Nanoparticles / Neoplasms, Experimental - immunology / Neoplasms, Experimental - therapy / Particle Size / Peptides / Polylysine - chemistry / Treatment Outcome / Tumors / Vaccines