Journal Article

Degradation of petroleum hydrocarbon contaminants by Rhodococcus erythropolis KB1 synergistic with alfalfa (Medicago sativa L.)

Zhu, Ning, Sun, Shangchen, Wang, Yonggang, Leng, Feifan, Lei, Tianzhu, Chen, Jixiang, Guo, Xiaopeng, Yang, Mingjun, Nan, Yan

2024

Overview

Petroleum hydrocarbons are a stubborn pollutant that is difficult to degrade globally, and plant-microbial degradation is the main way to solve this type of pollutant. In this study, the physiological and ecological responses of alfalfa to petroleum hydrocarbons in different concentrations of petroleum hydrocarbon-contaminated soil with KB1 ( Rhodococcus erythropolis ) were analyzed and determined by laboratory potting techniques. The growth of alfalfa (CK) and alfalfa with KB1 (JZ) in different concentrations of petroleum hydrocarbons contaminated soil was compared and analyzed. The results of the CK group showed that petroleum hydrocarbons could significantly affect the activity of alfalfa antioxidant enzyme system, inhibit the development of alfalfa roots and the normal growth of plants, especially in the high-concentration group. KB1 strain had the ability to produce IAA, form biofilm, fix nitrogen, produce betaine and ACC deaminase, and the addition of KB1 could improve the growth traits of alfalfa in the soil contaminated with different concentrations of petroleum hydrocarbons, the content of soluble sugars in roots, and the stress resistance and antioxidant enzyme activities of alfalfa. In addition, the degradation kinetics of the strain showed that the degradation rate of petroleum could reach 75.2% after soaking with KB1. Furthermore, KB1 can efficiently degrade petroleum hydrocarbons in advance and significantly alleviate the damage of high concentration of petroleum hydrocarbons to plant roots. The results showed that KB1 strains and alfalfa plants could effectively enhance the degradation of petroleum hydrocarbons, which provided new ideas for improving bioremediation strategies.

• Publisher: Springer Berlin Heidelberg,Springer Nature B.V
• Subject: 1-aminocyclopropane-1-carboxylate deaminase / Alfalfa / antioxidant enzymes / Antioxidants / Aquatic Pollution / Atmospheric Protection/Air Quality Control/Air Pollution / betaine / Biodegradation / Biodegradation, Environmental / biofilm / Biofilms / Bioremediation / Contaminants / Degradation / Earth and Environmental Science / Ecotoxicology / Environment / Environmental Chemistry / Environmental Health / Enzymatic activity / Enzymes / Hydrocarbons / Hydrocarbons - metabolism / Medicago sativa / Microbial degradation / Microorganisms / nitrogen / Petroleum / Petroleum - metabolism / Petroleum hydrocarbons / Plant growth / Plant roots / Plant Roots - metabolism / Pollutants / Research Article / Rhodococcus / Rhodococcus - metabolism / Rhodococcus erythropolis / Roots / soil / Soil contamination / Soil Microbiology / Soil Pollutants - metabolism / Soil pollution / stress tolerance / Waste Water Technology / Water Management / Water Pollution Control