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Expression and Cellular Distribution Pattern of Plasma Membrane Calcium Pump Isoforms in Rat Pancreatic Islets

by Caride, A.J. , Filoteo, A.G. , Del Zotto, H. , Penniston, J.T. , Rossi, J.P.F.C. , Gagliardino, J.J. , Garc a, M.E.

in Animals / Calcium-Transporting ATPases - metabolism / Cation Transport Proteins / Cell Membrane - metabolism / Culture Techniques / Islets of Langerhans - cytology / Islets of Langerhans - enzymology / Islets of Langerhans - metabolism / Isoenzymes - metabolism / Male / Plasma Membrane Calcium-Transporting ATPases / Rats / Rats, Wistar / Tissue Distribution

2002

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Expression and Cellular Distribution Pattern of Plasma Membrane Calcium Pump Isoforms in Rat Pancreatic Islets

by Caride, A.J. , Filoteo, A.G. , Del Zotto, H. , Penniston, J.T. , Rossi, J.P.F.C. , Gagliardino, J.J. , Garc a, M.E.

2002

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Expression and Cellular Distribution Pattern of Plasma Membrane Calcium Pump Isoforms in Rat Pancreatic Islets

by Caride, A.J. , Filoteo, A.G. , Del Zotto, H. , Penniston, J.T. , Rossi, J.P.F.C. , Gagliardino, J.J. , Garc a, M.E.

2002

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Journal Article

Expression and Cellular Distribution Pattern of Plasma Membrane Calcium Pump Isoforms in Rat Pancreatic Islets

Caride, A.J.,

Filoteo, A.G.,

Del Zotto, H.,

Penniston, J.T.,

Rossi, J.P.F.C.,

Gagliardino, J.J.,

Garc a, M.E.

2002

Overview

This work is aimed at identifying the presence and cellular distribution pattern of plasma membrane calcium pump (PMCA) isoforms in normal rat pancreatic islet. Microsomal fractions of isolated islets and exocrine tissue were analyzed to detect different PMCA isoforms. The cellular distribution pattern of these PMCAs in the islets was also studied in fixed pancreas sections incubated with antibodies against PMCAs and insulin. Antibody 5F10, which reacts with all PMCA variants, showed multiple bands in the blots in the 127-134 kDa region, indicating the presence of several isoforms. Microsomes also reacted positively with specific antibodies for individual PMCA isoforms, generating a band of the expected size. Antibody 5F10 immunocytochemically labeled the plasma cell membrane of both b- and non-b-cells, but predominantly the former. All islet cells were also labeled with antibodies against isoforms 1 and 4, while the antibody reacting with isoform 3 labeled exclusively b-cells. A few b- and non-b-cells were positively labeled with the antibody reacting with PMCA b variant. Negative results were obtained with the antibody against isoform 2. Further studies, together with previous reports on the modulatory effect of insulin secretagogues and blockers upon PMCA activity, may provide evidence of the importance of this particular PMCA expression for islet function under normal and pathological conditions.

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Publisher

Springer Nature B.V

Subject

Animals

/ Calcium-Transporting ATPases - metabolism

/ Cation Transport Proteins

/ Cell Membrane - metabolism

/ Culture Techniques

/ Islets of Langerhans - cytology

/ Islets of Langerhans - enzymology