Journal Article

Characterization and Separation of Cancer Cells with a Wicking Fiber Device

Tabbaa, Suzanne M., Burg, Karen J. L., Sharp, Julia L.

2017

Overview

Current cancer diagnostic methods lack the ability to quickly, simply, efficiently, and inexpensively screen cancer cells from a mixed population of cancer and normal cells. Methods based on biomarkers are unreliable due to complexity of cancer cells, plasticity of markers, and lack of common tumorigenic markers. Diagnostics are time intensive, require multiple tests, and provide limited information. In this study, we developed a novel wicking fiber device that separates cancer and normal cell types. To the best of our knowledge, no previous work has used vertical wicking of cells through fibers to identify and isolate cancer cells. The device separated mouse mammary tumor cells from a cellular mixture containing normal mouse mammary cells. Further investigation showed the device separated and isolated human cancer cells from a heterogeneous mixture of normal and cancerous human cells. We report a simple, inexpensive, and rapid technique that has potential to identify and isolate cancer cells from large volumes of liquid samples that can be translated to on-site clinic diagnosis.

• Publisher: Springer US,Springer Nature B.V
• Subject: Animals / Biochemistry / Biological and Medical Physics / Biomarkers / Biomedical and Life Sciences / Biomedical Engineering and Bioengineering / Biomedicine / Biophysics / Breast - cytology / Breast cancer / Cancer / Cell Line / Cell Separation - instrumentation / Classical Mechanics / Diagnostic systems / Equipment Design / Equipment Failure Analysis / Fibers / Mammary gland / Mice / Nanofibers - chemistry / Nanofibers - ultrastructure / Neoplastic Cells, Circulating - pathology / Polyesters - chemistry / Tumor cells / Tumors / Ultrafiltration - instrumentation