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Discrimination between sheep-associated and wildebeest-associated malignant catarrhal fever virus by means of a single-tube duplex nested PCR
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Discrimination between sheep-associated and wildebeest-associated malignant catarrhal fever virus by means of a single-tube duplex nested PCR
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Journal Article
Discrimination between sheep-associated and wildebeest-associated malignant catarrhal fever virus by means of a single-tube duplex nested PCR
2005
Overview
A single-tube duplex nested polymerase chain reaction (sdn-PCR) was developed for the detection of and discrimination between ovine herpesvirus-2 (OvHV-2) and alcelaphine herpesvirus-1 (AlHV-1). These viruses respectively cause sheep- and wildebeest-associated malignant catarrhal fever (SAMCF and WA-MCF). In the first step of the sdn-PCR, two primers with high annealing temperatures based on conserved regions of the tegument genes were used for DNA amplification. In the second step, two primer sets based on variable regions of the respective OvHV-2 and AlHV-1 genes and with annealing temperatures>11 °C below the primers used in the first step, were used. Internal regions of different sizes from amplicons produced in the first step were amplified. This single-tube test obviates the need for two separate assays to detect both viral types, thereby reducing time, labour and cost.
Publisher
AOSIS,AOSIS (Pty) Ltd
Subject
/ Animals
/ ANTILOPE
/ Herpesviridae - isolation & purification
/ HERPESVIRUS CORYZA GANGRENEUX
/ Malignant Catarrh - diagnosis
/ MALIGNANT CATARRHAL FEVER VIRUS
/ Malignant catarrhal fever viruses
/ Nucleic Acid Amplification Techniques - veterinary
/ OVIN
/ OVINOS
/ PCR
/ Polymerase Chain Reaction - methods
/ Polymerase Chain Reaction - veterinary
/ SHEEP
