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Effect of Lipoprotein-X on lipid metabolism in rat kidney
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Effect of Lipoprotein-X on lipid metabolism in rat kidney
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Journal Article
Effect of Lipoprotein-X on lipid metabolism in rat kidney
1997
Overview
Lipoprotein-X (Lp-X) is found in the plasma of patients with familial lecithin: cholesterol acyltransferase (LCAT) deficiency syndromes. The majority of the patients with this disorder develop progressive glomerulosclerosis. In this study, the effect of Lp-X on lipid metabolism in perfused rat kidney was investigated. Lp-X was isolated from plasma of patients with familial LCAT deficiency by sequential ultracentrifugation and gel filtration column chromatography. Rat kidneys were perfused for 1-2 h with Krebs-Henseleit buffer containing 20 microM [1-(14)C]acetate or 20 microM [Me-3H]choline. In the presence of Lp-X, no significant difference in the incorporation of radioactivity into triglycerides, cholesterol, phosphocholine, CDP-choline and sphingomyelin was observed. However, incorporation of radioactivity into cholesteryl esters and phosphatidylcholine was significantly elevated in Lp-X perfused kidneys. The contents of cholesterol, cholesteryl esters and phosphatidylcholine were also significantly increased in Lp-X perfused kidneys. The increase in lipid content in the Lp-X perfused kidney is attributed to the direct deposition of Lp-X lipids into the organ. The increase in the labelling of cholesteryl esters was attributed to the increase of available substrate (cholesterol) for the acyl-CoA:cholesterol acyltransferase (ACAT) reaction. The increase in phosphatidylcholine labelling was caused by a reduced turnover of the newly synthesized labelled phosphatidylcholine during Lp-X perfusion.
Publisher
Springer Nature B.V
Subject
1-Acylglycerophosphocholine O-Acyltransferase - drug effects
/ 1-Acylglycerophosphocholine O-Acyltransferase - metabolism
/ Animals
/ Esters
/ Humans
/ Kidneys
/ Lecithin Cholesterol Acyltransferase Deficiency - metabolism
/ Lipids
/ Lipoprotein-X - administration & dosage
/ Lipoprotein-X - pharmacology
/ Male
/ Phospholipases A - drug effects
/ Phospholipases A - metabolism
/ Rats
/ Rodents
