Asset Details
Do you wish to reserve the book?
Antioxidant metabolism and oxidative damage in Anthemis gilanica cell line under fast clinorotation
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Antioxidant metabolism and oxidative damage in Anthemis gilanica cell line under fast clinorotation
Do you wish to request the book?
Antioxidant metabolism and oxidative damage in Anthemis gilanica cell line under fast clinorotation
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Antioxidant metabolism and oxidative damage in Anthemis gilanica cell line under fast clinorotation
2022
Overview
Clinostat is a device often used for applying microgravity analogs in gravitational biology studies. It can constantly change the direction of the gravity vector through clinorotation with the possibility of speed regulation. In the present study, the impact of clinorotation (speed of 30 rpm) was investigated on the cell growth, viability, oxidative damage, and antioxidant compounds in Anthemis gilanica cell line using a two-dimensional clinostat. Cell clones were obtained from putting a cell suspension (1 ml) on the solid MS medium supplemented with 1-naphthaleneacetic acid (NAA, 0.5 mg L−1) and 6-benzylaminopurine (BAP, 1.5 mg L−1). Then, a cell line clone was transferred to a liquid MS medium for proliferation. The optimum subculture time for the cell line was found to be 14–15 days, and the cell line showed higher fresh weight compared to the control. The cell line was exposed to clinorotation (30 rpm) for 3 and 7 days. Results showed the cell growth parameters are managed with the ROS level and accumulation of antioxidant compounds in a time-dependent manner. Clinorotation increased cell growth, viability, protein content, DPPH, and antioxidant power, especially after 7 days. The level of H2O2, MDA, and protein-carbonyl increased after 3 days of clinorotation and then did not change significantly after 7 days compared to the control. Comet assay showed the weak genotoxicity impact of clinorotation on DNA damage in A. gilanica, which may relate to induction of antioxidant compounds (phenol and flavonoid) and PAL activity, especially after 7 days. But anthocyanin content did not change between treatments. The results display the potential effect of clinorotation on enhancing antioxidant capacity against oxidative damage and maintaining cell growth.Key messageCell line with a homogenous population of proliferating cells can prepare the proper situation to study various cell responses under microgravity. Improving of potential antioxidant by clinorotation mitigates oxidative damage in medicinal plant cells.
We currently cannot retrieve any items related to this title. Kindly check back at a later time.