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The phloem-pd: a distinctive brief sieve element stylet puncture prior to sieve element phase of aphid feeding behavior
by
Walker, G. P.
, Garzo, E.
, Alba-Tercedor, J.
, Jiménez, J.
, Moreno, A.
, Fereres, A.
in
Aphidoidea
/ Beet yellows virus
/ Behavioral Sciences
/ Beta vulgaris
/ Biomedical and Life Sciences
/ bundle sheath cells
/ Computed tomography
/ cryopreservation
/ Ecology
/ electric potential difference
/ Entomology
/ Feeding behavior
/ Inoculation
/ Invertebrates
/ Life Sciences
/ Mesophyll
/ micro-computed tomography
/ Myzus persicae
/ Original Paper
/ Parenchyma
/ parenchyma (plant tissue)
/ Phloem
/ Plant Pathology
/ Plant Sciences
/ Scanning microscopy
/ Sheaths
/ sieve elements
/ stylets
/ sugar beet
/ Viruses
/ Voltage
/ Voltage drop
/ Yellows
2020
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The phloem-pd: a distinctive brief sieve element stylet puncture prior to sieve element phase of aphid feeding behavior
by
Walker, G. P.
, Garzo, E.
, Alba-Tercedor, J.
, Jiménez, J.
, Moreno, A.
, Fereres, A.
in
Aphidoidea
/ Beet yellows virus
/ Behavioral Sciences
/ Beta vulgaris
/ Biomedical and Life Sciences
/ bundle sheath cells
/ Computed tomography
/ cryopreservation
/ Ecology
/ electric potential difference
/ Entomology
/ Feeding behavior
/ Inoculation
/ Invertebrates
/ Life Sciences
/ Mesophyll
/ micro-computed tomography
/ Myzus persicae
/ Original Paper
/ Parenchyma
/ parenchyma (plant tissue)
/ Phloem
/ Plant Pathology
/ Plant Sciences
/ Scanning microscopy
/ Sheaths
/ sieve elements
/ stylets
/ sugar beet
/ Viruses
/ Voltage
/ Voltage drop
/ Yellows
2020
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The phloem-pd: a distinctive brief sieve element stylet puncture prior to sieve element phase of aphid feeding behavior
by
Walker, G. P.
, Garzo, E.
, Alba-Tercedor, J.
, Jiménez, J.
, Moreno, A.
, Fereres, A.
in
Aphidoidea
/ Beet yellows virus
/ Behavioral Sciences
/ Beta vulgaris
/ Biomedical and Life Sciences
/ bundle sheath cells
/ Computed tomography
/ cryopreservation
/ Ecology
/ electric potential difference
/ Entomology
/ Feeding behavior
/ Inoculation
/ Invertebrates
/ Life Sciences
/ Mesophyll
/ micro-computed tomography
/ Myzus persicae
/ Original Paper
/ Parenchyma
/ parenchyma (plant tissue)
/ Phloem
/ Plant Pathology
/ Plant Sciences
/ Scanning microscopy
/ Sheaths
/ sieve elements
/ stylets
/ sugar beet
/ Viruses
/ Voltage
/ Voltage drop
/ Yellows
2020
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The phloem-pd: a distinctive brief sieve element stylet puncture prior to sieve element phase of aphid feeding behavior
Journal Article
The phloem-pd: a distinctive brief sieve element stylet puncture prior to sieve element phase of aphid feeding behavior
2020
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Overview
A recent electrical penetration graph (EPG) study identified a unique intracellular puncture that is associated with inoculation of the semipersistently transmitted, phloem-limited
Beet yellows virus
(BYV,
Closterovirus
) by the aphid
Myzus persicae
. This new aphid EPG pattern (named phloem-pd) always occurs shortly before phloem sieve element phase (PSEP) and has a similar voltage drop as PSEP, both of which are less than the voltage drop of standard-pds. Structure of pd subphase II-2 differs between phloem-pds and standard-pds. The objective of this study was to determine the type of phloem cell penetrated during phloem-pds. Stylets of
M. persicae
feeding on sugar beet (
Beta vulgaris
) were fixed in situ by cryofixation during phloem-pds, standard-pds and PSEP waveform E1. The cell penetrated by the stylet tips was then identified by confocal laser-scanning microscopy and micro-computed tomography. Sieve elements (SEs) or companion cells (CCs) were penetrated during phloem-pds, whereas cells other than SEs or CCs (mesophyll, bundle sheath cells and possibly phloem parenchyma) were penetrated during standard-pds. SEs were penetrated during waveform E1. The implications of these findings for inoculation of other phloem-limited viruses that currently are thought to be inoculated primarily during waveform E1 are discussed.
Publisher
Springer Netherlands,Springer Nature B.V
Subject
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