Journal Article

Pathogenesis-Related Protein 10 Family Genes Involved in Sugarcane Responses to Biotic Stressors and Salicylic Acid

Gao, San-Ji, Jiang, Xia-Ran, Lin, Jia-Xin, Zhao, Jian-Ying, Xu, Liang-Nian, Li, Juan, Javed, Talha

2024

Overview

Pathogenesis-related protein 10 (PR10) family genes play a crucial role in plant defense against various stressors. However, gene member and function identification of PR10 family in sugarcane remain largely unexplored. This study systematically identified and characterized this family genes in Saccharum spontaneum genome as well as their transcriptional expression in two sugarcane cultivars in response to infection by pathogenic bacteria Xanthomonas albilineans ( Xa ) and Acidovorax avenae subsp. avenae ( Aaa ) and foliar application of exogenous salicylic acid (SA). A total of 24 SsPR10 family genes were identified and categorized into two subfamilies, i.e., IPR10 ( SsIPR10-1–14 ) and NCS ( SsNCS-1–10 ). Six and two sets of SsPR10 underwent tandem and fragmental duplication events, respectively. RNA sequencing dataset and quantitative RT-PCR assay uncovered that ShIPR10-1/2/4/7/11/12 and ShNCS-8 genes were dramatically upregulated with increases of 3.2–107.2-fold in LCP85-384 (resistant to leaf scald), while they were slightly upregulated with increases of 1.26–7.1-fold in ROC20 (susceptible to leaf scald) for 24–72 h post inoculation under Xa infection. ShIPR10-1/7/12 and ShNCS-8 were significantly upregulated in both cultivars triggered by Aaa infection with increases of 2.4–21.0-fold. Under exogenous salicylic acid treatment, ShIPR10-1/2/4/7/11/12 were remarkably upregulated by 3.6–6.1-fold in LCP85-384 at 12 h post treatment (hpt) but were downregulated by 81.3% in ROC20 at 24 hpt, as compared to the control 0 hpt. In addition, ShNPR1 and ShPR1 involved in the SA signal transduction pathway displayed positive roles in sugarcane under both bacterial attacks and SA treatment. Collectively, our data provide important genetic resources for improving sugarcane tolerance to pathogenic bacterial stress and disease-resistance breeding in sugarcane for food security.

• Publisher: Springer US,Springer Nature B.V
• Subject: acid treatment / Acidovorax avenae avenae / Agriculture / Bacteria / Biomedical and Life Sciences / Cultivars / data collection / Disease resistance / family / foliar application / Foliar applications / Food security / Gene sequencing / Genes / Genetic resources / Infections / Inoculation / Leaves / Life Sciences / Pathogenesis / pathogenesis-related proteins / Plant Anatomy/Development / Plant bacterial diseases / Plant breeding / plant growth / Plant Physiology / Plant Sciences / Proteins / RNA / Saccharum spontaneum / Salicylic acid / Scald / scald diseases / Signal transduction / Sugarcane / transcription (genetics) / Xanthomonas albilineans