Asset Details
Do you wish to reserve the book?
Molecular Expression Differences in Specific Blood Mononuclear Cell‐Types Identify Patients With AL Amyloidosis
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Molecular Expression Differences in Specific Blood Mononuclear Cell‐Types Identify Patients With AL Amyloidosis
Do you wish to request the book?
Molecular Expression Differences in Specific Blood Mononuclear Cell‐Types Identify Patients With AL Amyloidosis
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Molecular Expression Differences in Specific Blood Mononuclear Cell‐Types Identify Patients With AL Amyloidosis
2025
Overview
The diagnosis of AL amyloidosis is often challenging due to its systemic nature and heterogeneous clinical presentation. Current serological biomarkers for diagnosis and monitoring are not optimal. We have considered the possibility that mononuclear cell‐type specific molecular expression can be used to develop blood‐based biomarkers to diagnose and monitor patients with AL amyloidosis. Peripheral blood monocytes and CD4+ T cells from patients with documented AL amyloidosis or myeloma without amyloidosis were assessed by enhanced flow cytometric analysis for expression levels of 20 analytes chosen for the possibility that their expression levels may lead to diagnostic assays and biomarkers. We found definitive expression level differences for brain‐derived neurotrophin factor (BDNF), calmodulin, and phospho‐TBK1 in CD4+ T cells and for phospho‐GSK3β in monocytes. Logistic regression and ROC analysis showed that BDNF in CD4+ T cells and heme oxygenase 1 in monocytes significantly distinguished between patients with myeloma versus patients with AL amyloidosis (AUC = 0.75). Additionally, we discovered remarkable differences in intermolecular associations between the samples from the two patient groups, suggesting the involvement of specific pathogenetic pathways. Our results demonstrate that mononuclear cell‐type specific molecular expression may be useful for developing a diagnostic assay and biomarkers for patients with AL amyloidosis.
Publisher
John Wiley & Sons, Inc
Subject
/ Biopsy
/ Brain-derived neurotrophic factor
/ CD4-Positive T-Lymphocytes - metabolism
/ Cells
/ cell‐specific molecular expression
/ Female
/ Heme oxygenase (decyclizing)
/ Humans
/ Immunoglobulin Light-chain Amyloidosis - blood
/ Immunoglobulin Light-chain Amyloidosis - diagnosis
/ Immunoglobulin Light-chain Amyloidosis - genetics
/ Leukocytes, Mononuclear - metabolism
/ Male
/ Multiple Myeloma - diagnosis
/ Myeloma
/ Software
