Journal Article

Effect of Sodium Thiosulfate Pre-Treatment on Renal Ischemia-Reperfusion Injury in Kidney Transplantation

Major, Sally, Nelson, Pierce, McFarlane, Liam, O’Neil, Caroline, Sener, Alp, McLeod, Patrick, Haig, Aaron, Jiang, Jifu, Dugbartey, George J.

2024

Overview

We recently reported in a rat model of kidney transplantation that the addition of sodium thiosulfate (STS) to organ preservation solution improved renal graft quality and prolonged recipient survival. The present study investigates whether STS pre-treatment would produce a similar effect. In vitro, rat kidney epithelial cells were treated with 150 μM STS before and/or during exposure to hypoxia followed by reoxygenation. In vivo, donor rats were treated with PBS or 2.4 mg/kg STS 30 min before donor kidneys were procured and stored in UW or UW+150 μM STS solution at 4 °C for 24 h. Renal grafts were then transplanted into bilaterally nephrectomised recipient rats which were then sacrificed on post-operative day 3. STS pre-treatment significantly reduced cell death compared to untreated and other treated cells in vitro (p < 0.05), which corresponded with our in vivo result (p < 0.05). However, no significant differences were observed in other parameters of tissue injury. Our results suggest that STS pre-treatment may improve renal graft function after transplantation.

• Publisher: MDPI AG
• Subject: Animals / Apoptosis / Cell death / Cold storage / Creatinine / Hypoxia / Inflammation / Ischemia / Kidney - drug effects / Kidney Transplantation - adverse effects / Kidney Transplantation - methods / Kidney transplants / Male / Organ Preservation - methods / Organ Preservation Solutions - pharmacology / Oxidative stress / Rats / Reperfusion Injury - drug therapy / Statistical analysis / Thiosulfates - pharmacology / Thiosulfates - therapeutic use / Urine