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Autotrophic cells of the Synechocystis psbH deletion mutant are deficient in synthesis of CP47 and accumulate inactive PS II core complexes
by
Komenda, J
in
Bacteria
/ bacterial proteins
/ biosynthesis
/ chemistry
/ chlorophyll-binding protein CP-47
/ Cyanobacteria
/ Cyanophyta
/ cytology
/ deficiency
/ Gene Deletion
/ genetics
/ Glucose
/ Glucose - metabolism
/ light harvesting complex
/ Light-Harvesting Protein Complexes
/ Light-Harvesting Protein Complexes - biosynthesis
/ metabolism
/ mutation
/ phosphoproteins
/ Phosphoproteins - deficiency
/ Phosphoproteins - genetics
/ Phosphoproteins - metabolism
/ photosynthetic reaction centers
/ photosystem II
/ Photosystem II Protein Complex
/ Photosystem II Protein Complex - biosynthesis
/ Photosystem II Protein Complex - chemistry
/ Photosystem II Protein Complex - genetics
/ Photosystem II Protein Complex - metabolism
/ protein synthesis
/ Synechocystis
/ Synechocystis - cytology
/ Synechocystis - genetics
/ Synechocystis - metabolism
/ Synechocystis sp. PCC 6803
2005
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Autotrophic cells of the Synechocystis psbH deletion mutant are deficient in synthesis of CP47 and accumulate inactive PS II core complexes
by
Komenda, J
in
Bacteria
/ bacterial proteins
/ biosynthesis
/ chemistry
/ chlorophyll-binding protein CP-47
/ Cyanobacteria
/ Cyanophyta
/ cytology
/ deficiency
/ Gene Deletion
/ genetics
/ Glucose
/ Glucose - metabolism
/ light harvesting complex
/ Light-Harvesting Protein Complexes
/ Light-Harvesting Protein Complexes - biosynthesis
/ metabolism
/ mutation
/ phosphoproteins
/ Phosphoproteins - deficiency
/ Phosphoproteins - genetics
/ Phosphoproteins - metabolism
/ photosynthetic reaction centers
/ photosystem II
/ Photosystem II Protein Complex
/ Photosystem II Protein Complex - biosynthesis
/ Photosystem II Protein Complex - chemistry
/ Photosystem II Protein Complex - genetics
/ Photosystem II Protein Complex - metabolism
/ protein synthesis
/ Synechocystis
/ Synechocystis - cytology
/ Synechocystis - genetics
/ Synechocystis - metabolism
/ Synechocystis sp. PCC 6803
2005
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Autotrophic cells of the Synechocystis psbH deletion mutant are deficient in synthesis of CP47 and accumulate inactive PS II core complexes
by
Komenda, J
in
Bacteria
/ bacterial proteins
/ biosynthesis
/ chemistry
/ chlorophyll-binding protein CP-47
/ Cyanobacteria
/ Cyanophyta
/ cytology
/ deficiency
/ Gene Deletion
/ genetics
/ Glucose
/ Glucose - metabolism
/ light harvesting complex
/ Light-Harvesting Protein Complexes
/ Light-Harvesting Protein Complexes - biosynthesis
/ metabolism
/ mutation
/ phosphoproteins
/ Phosphoproteins - deficiency
/ Phosphoproteins - genetics
/ Phosphoproteins - metabolism
/ photosynthetic reaction centers
/ photosystem II
/ Photosystem II Protein Complex
/ Photosystem II Protein Complex - biosynthesis
/ Photosystem II Protein Complex - chemistry
/ Photosystem II Protein Complex - genetics
/ Photosystem II Protein Complex - metabolism
/ protein synthesis
/ Synechocystis
/ Synechocystis - cytology
/ Synechocystis - genetics
/ Synechocystis - metabolism
/ Synechocystis sp. PCC 6803
2005
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Autotrophic cells of the Synechocystis psbH deletion mutant are deficient in synthesis of CP47 and accumulate inactive PS II core complexes
Journal Article
Autotrophic cells of the Synechocystis psbH deletion mutant are deficient in synthesis of CP47 and accumulate inactive PS II core complexes
2005
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Overview
Cells of the psbH deletion mutant IC7 of the cyanobacterium Synechocystis PCC 6803 grown in the absence of glucose contain strongly reduced levels of chlorophyll when compared with cells grown in the presence of glucose, or compared with wild-type (WT) cells. Low-temperature fluorescence emission spectra revealed decreased content of both active PS II (Photosystem II) and PS I (Photosystem I) complexes. Analysis of thylakoid membrane complexes of IC7 by native electrophoresis showed a similar set of chlorophyll-proteins, namely a PS II core complex and trimeric and monomeric PS II complexes, as in WT. However, in contrast to WT, the (35)S-methionine protein labeling pattern of the mutant exhibited no preferential labeling of the D1 protein in the PS II core complexes, and the labeled D1 and D2 proteins accumulated predominantly in the PS II reaction center lacking CP47. The results show that in autotrophically grown cells of the psbH deletion mutant, selective D1 turnover is inhibited and synthesis of CP47 becomes a limiting step in the PS II assembly.
Publisher
Springer Nature B.V
Subject
/ chlorophyll-binding protein CP-47
/ cytology
/ genetics
/ Glucose
/ Light-Harvesting Protein Complexes
/ Light-Harvesting Protein Complexes - biosynthesis
/ mutation
/ Phosphoproteins - deficiency
/ Phosphoproteins - metabolism
/ photosynthetic reaction centers
/ Photosystem II Protein Complex
/ Photosystem II Protein Complex - biosynthesis
/ Photosystem II Protein Complex - chemistry
/ Photosystem II Protein Complex - genetics
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