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Production of derivatives of α-terpineol by bacterial CYP102A1 enzymes
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Production of derivatives of α-terpineol by bacterial CYP102A1 enzymes
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Journal Article
Production of derivatives of α-terpineol by bacterial CYP102A1 enzymes
2025
Overview
The monooxygenase activity of engineered CYP102A1 on α-terpineol was investigated. CYP102A1 M850 mutant (F11Y/R47L/D68G/F81I/F87V/E143G/L188Q/E267V/H408R) showed the highest catalytic activity toward α-terpineol among the engineered mutants produced by random mutagenesis. The major product (P1) of α-terpineol,
p
-menth-1-ene-3,8-diol, was characterized by high-performance liquid chromatography, gas-chromatography mass spectrometry, and nuclear magnetic resonance spectroscopy. Three minor products (P2–P4) of α-terpineol were considered as 6-hydroxy-α,α,4-trimethyl-3-cyclohexene-1-methanol (P2),
trans
-sobrerol (P3), and carvone hydrate (P4). Optimal conditions for product formation were determined as pH 7.0 and 30 °C. Production of
p
-menth-1-ene-3,8-diol was 0.87 mM at 1 h. Structure modeling using PyMOL and CAVER Web 1.2 server indicated that several mutations of CYP102A1 M850 were involved in access tunnels and active sites, resulting in increased activity toward α-terpineol. The major product,
p
-menth-1-ene-3,8-diol, of α-terpineol was produced by engineered CYP102A1 M850 via regioselective carbon hydroxylation. The engineered CYP102A1 could be a suitable biocatalyst for producing α-terpineol derivatives.
Publisher
Springer Netherlands,Springer Nature B.V
Subject
/ Bacterial Proteins - chemistry
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Biomedical and Life Sciences
/ carbon
/ Carvone
/ Cyclohexane Monoterpenes - chemistry
/ Cyclohexane Monoterpenes - metabolism
/ Cytochrome P-450 Enzyme System - chemistry
/ Cytochrome P-450 Enzyme System - genetics
/ Cytochrome P-450 Enzyme System - metabolism
/ Enzymes
/ Glucose
/ High-performance liquid chromatography
/ Magnetic resonance spectroscopy
/ Mutants
/ NADPH-Ferrihemoprotein Reductase - chemistry
/ NADPH-Ferrihemoprotein Reductase - genetics
/ NADPH-Ferrihemoprotein Reductase - metabolism
/ NMR
