Journal Article

Pollen Micromorphology among Amaranthaceous Species from Desert Rangeland: Exine Stratification and their Taxonomic Significance

Zafar, Muhammad, Ali, Alamdar, Gafforov, Yusufjon, Kilic, Omer, Makhkamov, Trobjon, Demirpolat, Azize, Afza, Rabia, Sultana, Shazia, Yuldashev, Akramjon, Khaydarov, Khislat, Ahmad, Mushtaq, Çobanoğlu, Duygu Nur, Zafar, Sadia, Althobaiti, Ashwaq T., Ramadan, Mohamed Fawzy, Majeed, Salman

2023

Overview

The aim of the study was to visualize the micromorphology of Amaranthaceous pollen using scanning electron microscopy collected from the Thal Desert. Field collection was conducted from July to September 2021. A total of 14 taxa of the family Amaranthaceae were collected which belong to nine genera. Achyranthes aspera, Aerva javanica, Aerva lanata, Amaranthus graecizans, Amaranthus retroflexus, Amaranthus viridis, Bassia indica, Chenopodium album, Chenopodium ficifolium, Chenopodium murale, Digera muricata, Haloxylon stocksii, Salsola tragus, and Suaeda fruticosa were studied in terms of pollen morphotypes. Pollen were acetolyzed and observed under optical and scanning microscopy. Qualitative and quantitative characters were measured to analyze the pollen to uncover its taxonomic significance. Qualitative characters observed were the shape of pollen in polar and equatorial views; the most frequent shape observed was spheroidal in the polar view, whereas in the equatorial view, prolate spheroidal was the dominant shape. Exine ornamentation is the key characteristic of pollen which is very helpful, and eight different types of ornamentations were observed in collected taxa: smooth sparsely granulate, scabrate-spinulose, microspinulose perforate, microechinate scabrate to metareticulate, granulate, nanospinulate, granulate-spinulose perforate, granulate-perforate echinate, and microechinate perforate. Periporate-type aperture was observed among all taxa. Mesoporia, ektexinous bodies, and tectum features also show variations among Amaranthaceous grains. In quantitative character, A. retroflexus recorded highest in polar view 26.3 μm and the lowest was of C. album 12.2 μm. Highest P/E index ratio was recorded in S. fruticosa (1.12) whereas the lowest for D. muricta (0.94). Exine thickness was highest in S. tragus 2.15 μm and lowest in A. graecizans 0.78 μm. The maximum number of pores was recorded as 32-36 in D. muricata. Artificial taxonomic keys were constructed based on findings that reinforce the importance of the micromorphological ultrastructural diversity of pollen among Amaranthaceous taxa. It was concluded that the descriptions of pollen morphotypes presented greatly contribute to our understanding of desert species identification.

• Publisher: Hindawi,John Wiley & Sons, Inc
• Subject: Amaranthaceae / Deserts / Environmental aspects / Flowers & plants / Genera / Granulation / Identification and classification / Morphology / Physiological aspects / Pollen / Qualitative analysis / Rangelands / Salsola tragus / Scanning electron microscopy / Scanning microscopy / Taxa / Taxonomic significance / Taxonomy