Journal Article

Escherichia coli adhesion protein FimH exacerbates colitis via CD11b+CD103- dendritic cell activation

Kim, So-Jung, Lee, Peter C. W., An, Eun-Koung, Zhang, Wei, Jin, Jun-O, Park, Hae-Bin

2023

Overview

Immune stimulators are used to improve vaccine efficiency; however, they are accompanied by various side effects. In previous studies, we reported that the adhesion protein, FimH, induces immune activity; however, we did not examine any side effects in colon inflammation. FimH was administered orally or intraperitoneally ( ) to mice with dextran sulfate sodium (DSS)-induced colitis, and changes in symptoms were observed. Immune cells infiltrated into the colon after the induction of colon inflammation were analyzed using a flow cytometer. Changes in Th1 and Th17 cells that induce colitis were analyzed. Further, mesenteric lymph node (mLN) dendritic cells (DCs) activated by FimH were identified and isolated to examine their ability to induce T-cell immunity. FimH oral and administration in C57BL/6 mice did not induce inflammation in the colon; however, DSS-induced colitis was exacerbated by oral and FimH administration. FimH treatment increased immune cell infiltration in the colon compared to that in DSS colitis. Th1 and Th17 cells, which are directly related to colitis, were increased in the colon by FimH; however, FimH did not directly affect the differentiation of these T cells. FimH upregulated the CD11b CD103 DC activity in the mLNs, which produced the signature cytokines required for Th1 and Th17. In addition, isolated CD11b CD103 DCs, after stimulation with FimH, directly induced Th1 and Th17 differentiation in a co-culture of CD4 T cells. This study demonstrated the side effects of FimH and indicated that the use of FimH can aggravate the disease in patients with colitis.

• Publisher: Frontiers Media SA,Frontiers Media S.A
• Subject: Animals / Antibodies / Antigens / CD103 antigen / CD11b antigen / CD4 antigen / Cell activation / Cell culture / Cell differentiation / Cells / Colitis / Colon / Crohn's disease / dendritic cell / Dendritic Cells / Dextran / Dextran sulfate / E coli / Escherichia coli / FimH / Helper cells / Humans / Immunity (Disease) / Inflammation / Inflammation - metabolism / Inflammatory bowel disease / Inflammatory diseases / Ligands / Lymph nodes / Lymphocytes / Lymphocytes T / Mice / Mice, Inbred C57BL / Oral administration / Proteins / Side effects / Th1 / Th17 / Vaccines