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Functional and structural alterations of epithelial barrier properties of rat ileum following X-irradiation
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Functional and structural alterations of epithelial barrier properties of rat ileum following X-irradiation
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Journal Article
Functional and structural alterations of epithelial barrier properties of rat ileum following X-irradiation
2004
Overview
Irradiation of the digestive system leads to alterations of the small intestine. We have characterized the disruption of the barrier integrity in rat ileum from 1 to 14 days following irradiation ranging from 6 to 12 Gy. The intestinal permeability to
14
C-mannitol and
3
H-dextran 70 000 was measured in vitro in Ussing chambers. In parallel to these functional studies, immunohistochemical analyses of junctional proteins (ZO-1 and β-catenin) of ileal epithelium were performed by confocal microscopy. Irradiation with 10 Gy induced a marked decrease in epithelial tissue resistance at three days and a fivefold increase in mannitol permeability, without modifications of dextran permeability. A disorganization of the localization for ZO-1 and β-catenin was also observed. At 7 days after irradiation, we observed a recovery of the organization of junctional proteins in parallel to a return of intestinal permeability to control value. In addition to these time-dependent effects, a gradual effect on epithelial integrity of the radiation doses was observed 3 days after irradiation. This study shows a disruption of the integrity of the intestinal barrier in rat ileum following abdominal X-irradiation, depending on the time postirradiation and on the delivered dose. The loss of barrier integrity was characterized by a disorganization of proteins of tight and adherent junctions, leading to increased intestinal permeability to mannitol.Key words: intestinal permeability, ZO-1, β-catenin, tight and adherent junctions.
Publisher
NRC Research Press,National Research Council of Canada
Subject
/ Biological and medical sciences
/ Cell Membrane Permeability - radiation effects
/ Dose-Response Relationship, Radiation
/ Fundamental and applied biological sciences. Psychology
/ Intercellular Junctions - metabolism
/ Intercellular Junctions - radiation effects
/ Intercellular Junctions - ultrastructure
/ Intestinal Mucosa - physiopathology
/ Intestinal Mucosa - radiation effects
/ Intestinal Mucosa - ultrastructure
/ Male
/ Radiation Injuries - pathology
/ Rats
