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Curcumin inhibits AP-2γ-induced apoptosis in the human malignant testicular germ cells in vitro
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Curcumin inhibits AP-2γ-induced apoptosis in the human malignant testicular germ cells in vitro
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Journal Article
Curcumin inhibits AP-2γ-induced apoptosis in the human malignant testicular germ cells in vitro
2013
Overview
Aim： To investigate the effects of curcumin on proliferation and apoptosis in testicular cancer cells in vitro and to investigate its molecular mechanisms of action.
Methods： NTera-2 human malignant testicular germ cell line and F9 mouse teratocarcinoma stem cell line were used. The anti-proliferative effect was examined using MTT and colony formation assays. Hoechst 33258 staining, TUNEL and Annexin V-FITC/PI staining assays were used to analyze cell apoptosis. Protein expression was examined with Western blot analysis and immunocytochemical staining.
Results： Curcumin （5, 10 and 15 μmol/L） inhibited the viability of NTera-2 cells in dose- and time-dependent manners. Curcumin significantly inhibited the colony formation in both NTera-2 and F9 cells. Curcumin dose-dependently induced apoptosis of NTera-2 cells by reducing FasL expression and Bcl-2-to-Bax ratio, and activating caspase-9, -8 and -3. Furthermore, curcumin dose-dependently reduced the expression of AP transcription factor AP-2γ in NTera-2 cells, whereas the pretreatment with the proteasome inhibitor MG132 blocked both the curcumin-induced reduction of AP-2γ and antiproliferative effect. Curcumin inhibited ErbB2 expression, and decreased the phosphorylation of Akt and ERK in NTera-2 cells.
Conclusion： Curcumin induces apoptosis and inhibits proliferation in NTera-2 cells via the inhibition of AP-2γ-mediated downstream cell survival signaling pathways.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Annexin
/ Antineoplastic Agents - pharmacology
/ Antineoplastic Agents - therapeutic use
/ Biomedical and Life Sciences
/ Cell Proliferation - drug effects
/ Humans
/ Mice
/ Neoplasms, Germ Cell and Embryonal - drug therapy
/ Neoplasms, Germ Cell and Embryonal - pathology
/ Original
/ Testicular Neoplasms - drug therapy
/ Testicular Neoplasms - pathology
/ Transcription Factor AP-2 - antagonists & inhibitors
/ Transcription Factor AP-2 - pharmacology
/ Vaccine
/ 体外诱导
/ 姜黄素
/ 恶性
/ 时间依赖性
/ 生精细胞
/ 睾丸
/ 细胞凋亡
