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EGR1 is crucial for the chlorogenic acid–provided promotion on liver regeneration and repair after APAP-induced liver injury
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EGR1 is crucial for the chlorogenic acid–provided promotion on liver regeneration and repair after APAP-induced liver injury
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Journal Article
EGR1 is crucial for the chlorogenic acid–provided promotion on liver regeneration and repair after APAP-induced liver injury
2023
Overview
Improper use of acetaminophen (APAP) will induce acute liver failure. This study is designed to investigate whether early growth response-1 (EGR1) participated in the promotion on liver repair and regeneration after APAP-induced hepatotoxicity provided by natural compound chlorogenic acid (CGA). APAP induced the nuclear accumulation of EGR1 in hepatocytes regulated by extracellular-regulated protein kinase (ERK)1/2. In
Egr1
knockout (KO) mice, the liver damage caused by APAP (300 mg/kg) was more severe than in wild-type (WT) mice. Results of chromatin immunoprecipitation and sequencing (ChIP-Seq) manifested that EGR1 could bind to the promoter region in
Becn1
,
Ccnd1
, and
Sqstm1
(
p62
) or the catalytic/modify subunit of glutamate-cysteine ligase (
Gclc
/
Gclm
). Autophagy formation and APAP-cysteine adduct (APAP-CYS) clearance were decreased in
Egr1
KO mice administered with APAP. The EGR1 deletion reduced hepatic cyclin D1 expression at 6, 12, or 18 h post APAP administration. Meanwhile, the EGR1 deletion also decreased hepatic p62, Gclc and Gclm expression, GCL enzymatic activity, and glutathione (GSH) content and decreased nuclear factor erythroid 2-related factor 2 (Nrf2) activation and thus aggravated oxidative liver injury induced by APAP. CGA increased EGR1 nuclear accumulation; enhanced hepatic
Ccnd1
,
p62
,
Gclc
, and
Gclm
expression; and accelerated the liver regeneration and repair in APAP-intoxicated mice. In conclusion, EGR1 deficiency aggravated liver injury and obviously delayed liver regeneration post APAP-induced hepatotoxicity through inhibiting autophagy, enhancing liver oxidative injury, and retarding cell cycle progression, but CGA promoted the liver regeneration and repair in APAP-intoxicated mice via inducing EGR1 transcriptional activation.
Graphical Abstract
Publisher
Springer Netherlands,Springer Nature B.V
Subject
/ Animals
/ Biomedical and Life Sciences
/ Chemical and Drug Induced Liver Injury - metabolism
/ Chemical and Drug Induced Liver Injury, Chronic - metabolism
/ Chemical and Drug Induced Liver Injury, Chronic - pathology
/ Chlorogenic Acid - metabolism
/ Chlorogenic Acid - pharmacology
/ Cysteine
/ Deletion
/ Injuries
/ Kinases
/ Liver
/ Mice
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