Asset Details
Do you wish to reserve the book?
DNA damage and repair in peripheral blood mononuclear cells after internal ex vivo irradiation of patient blood with 131I
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
DNA damage and repair in peripheral blood mononuclear cells after internal ex vivo irradiation of patient blood with 131I
Do you wish to request the book?
DNA damage and repair in peripheral blood mononuclear cells after internal ex vivo irradiation of patient blood with 131I
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
DNA damage and repair in peripheral blood mononuclear cells after internal ex vivo irradiation of patient blood with 131I
2022
Overview
Aim
The aim of this study was to provide a systematic approach to characterize DNA damage induction and repair in isolated peripheral blood mononuclear cells (PBMCs) after internal ex vivo irradiation with [
131
I]NaI. In this approach, we tried to mimic ex vivo the irradiation of patient blood in the first hours after radioiodine therapy.
Material and methods
Blood of 33 patients of two centres was collected immediately before radioiodine therapy of differentiated thyroid cancer (DTC) and split into two samples. One sample served as non-irradiated control. The second sample was exposed to ionizing radiation by adding 1 ml of [
131
I]NaI solution to 7 ml of blood, followed by incubation at 37 °C for 1 h. PBMCs of both samples were isolated, split in three parts each and (i) fixed in 70% ethanol and stored at − 20 °C directly (0 h) after irradiation, (ii) after 4 h and (iii) 24 h after irradiation and culture in RPMI medium. After immunofluorescence staining microscopically visible co-localizing γ-H2AX + 53BP1 foci were scored in 100 cells per sample as biomarkers for radiation-induced double-strand breaks (DSBs).
Results
Thirty-two of 33 blood samples could be analysed. The mean absorbed dose to the blood in all irradiated samples was 50.1 ± 2.3 mGy. For all time points (0 h, 4 h, 24 h), the average number of γ-H2AX + 53BP1 foci per cell was significantly different when compared to baseline and the other time points. The average number of radiation-induced foci (RIF) per cell after irradiation was 0.72 ± 0.16 at
t
= 0 h, 0.26 ± 0.09 at
t
= 4 h and 0.04 ± 0.09 at
t
= 24 h. A monoexponential fit of the mean values of the three time points provided a decay rate of 0.25 ± 0.05 h
−1
, which is in good agreement with data obtained from external irradiation with γ- or X-rays.
Conclusion
This study provides novel data about the ex vivo DSB repair in internally irradiated PBMCs of patients before radionuclide therapy. Our findings show, in a large patient sample, that efficient repair occurs after internal irradiation with 50 mGy absorbed dose, and that the induction and repair rate after
131
I exposure is comparable to that of external irradiation with γ- or X-rays.
