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Extraction of activated sludge bacteria exopolymers by ultrasonication
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Extraction of activated sludge bacteria exopolymers by ultrasonication
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Journal Article
Extraction of activated sludge bacteria exopolymers by ultrasonication
2003
Overview
Ultrasonication for the extraction of activated sludge exopolymers was evaluated by total cell count, exopolymer extraction and transmission electron microscopy (TEM). A high deflocculation was achieved after 30 s of sonication in PBS (phosphate-buffered saline). TEM showed that cell lysis was minimal only when sludges were sonicated for 30 s. For sludges sonicated for 30, 90 and 420 s and stained with Ruthenium Red, exopolymers were not extracted on a large scale without considerable cell lysis. Sludges sonicated for 30 s in EDTA gave a larger fraction of damaged cells and also showed copious amounts of attached exopolymers.
Publisher
Springer Nature B.V
Subject
/ Bacteria
/ Bacteria, Aerobic - growth & development
/ Bacteria, Aerobic - metabolism
/ Bacteria, Aerobic - radiation effects
/ Bacteria, Aerobic - ultrastructure
/ Biofilms - growth & development
/ Biofilms - radiation effects
/ Cell Aggregation - radiation effects
/ Cell Culture Techniques - methods
/ Cell Division - radiation effects
/ Dose-Response Relationship, Radiation
/ Polysaccharides, Bacterial - biosynthesis
