Asset Details
Do you wish to reserve the book?
miR-142-3p promotes osteoblast differentiation by modulating Wnt signaling
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
miR-142-3p promotes osteoblast differentiation by modulating Wnt signaling
Do you wish to request the book?
miR-142-3p promotes osteoblast differentiation by modulating Wnt signaling
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
miR-142-3p promotes osteoblast differentiation by modulating Wnt signaling
2013
Overview
Canonical Wnt signaling is critical for the control of osteoblast differentiation in human mesenchymal stem cells. MicroRNAs (miRs) are essential regulators of cell differentiation by post-transcriptional regulation of target gene expression. The aim of the present study was to investigate the molecular mechanism by which miR-142-3p promotes osteoblastic differentiation using the human fetal osteoblastic 1.19 (hFOB1.19), real-time PCR and western blot analysis. Results showed an increased expression of miR-142-3p during osteoblast differentiation in the mesenchymal precursor cell line, hFOB1.19. In addition, the ectopic overexpression of miR-142-3p promoted hFOB1.19 differentiation, whereas the inhibition of miR-142-3p repressed differentiation. The expression of miR-142-3p was positively correlated with β-catenin, an important protein in Wnt signaling. The adenomatous polyposis coli (APC) gene was a direct target of miR-142-3p, whereby miR-142-3p promoted Wnt signaling through inhibition of APC, leading to accumulation and nuclear translocation of β-catenin. Therefore, miR-142-3p may be an essential mediator of osteoblast differentiation and a new therapeutic strategy for osteogenesis disorders.
Publisher
D.A. Spandidos,Spandidos Publications UK Ltd
Subject
/ Adenomatous polyposis coli protein
/ Adenomatous Polyposis Coli Protein - antagonists & inhibitors
/ Adenomatous Polyposis Coli Protein - genetics
/ Adenomatous Polyposis Coli Protein - metabolism
/ APC
/ Embryos
/ Fetuses
/ Humans
/ Kinases
/ miRNA
/ Oligonucleotides, Antisense - genetics
/ Oligonucleotides, Antisense - metabolism
/ Rodents
/ Wnt
