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From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction
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From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction
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Journal Article
From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction
2022
Overview
The central nervous system (CNS) controls and regulates the functional activities of the organ systems and maintains the unity between the body and the external environment. The advent of co-culture systems has made it possible to elucidate the interactions between neural cells in vitro and to reproduce complex neural circuits. Here, we classified the co-culture system as a two-dimensional (2D) co-culture system, a cell-based three-dimensional (3D) co-culture system, a tissue slice-based 3D co-culture system, an organoid-based 3D co-culture system, and a microfluidic platform-based 3D co-culture system. We provide an overview of these different co-culture models and their applications in the study of neural cell interaction. The application of co-culture systems in virus-infected CNS disease models is also discussed here. Finally, the direction of the co-culture system in future research is prospected.
Publisher
MDPI AG,MDPI
Subject
/ Review
