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In Vivo Activity and Duration of Short Interfering RNAs Containing a Synthetic 5′-Phosphate
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In Vivo Activity and Duration of Short Interfering RNAs Containing a Synthetic 5′-Phosphate
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Journal Article
In Vivo Activity and Duration of Short Interfering RNAs Containing a Synthetic 5′-Phosphate
2012
Overview
Endogenous and exogenous short interfering RNAs (siRNAs) require a 5′-phosphate for loading into Ago2 and cleavage of the target mRNA. We applied a synthetic 5′-phosphate to siRNA guide strands to evaluate if phosphorylation
in vivo
is rate limiting for maximal siRNA knockdown and duration. We report, for the first time, an
in vivo
evaluation of siRNAs with a synthetic 5′-phosphate compared to their unphosphorylated versions. siRNAs that contained a 5′-phosphate had the same activity
in vivo
compared with unphosphorylated siRNAs, indicating phosphorylation of an siRNA is not a rate limiting step
in vivo
.
Publisher
Mary Ann Liebert, Inc,SAGE Publications
