Journal Article

Punicalagin ameliorates collagen-induced arthritis by downregulating M1 macrophage and pyroptosis via NF-κB signaling pathway

Xue, Yi, Tao, Huaqiang, Wei, Minggang, Xu, Yaozeng, Niu, Junjie, Bai, Jiaxiang, Wang, Qing, Ge, Gaoran, Yang, Huilin, Geng, Dechun, Hao, Yuefeng, Liang, Xiaolong, Chen, Hao

2022

Overview

Rheumatoid arthritis (RA) is a chronic inflammatory disease that eventually leads to disability. Inflammatory cell infiltration, severe joint breaking and systemic bone loss are the main clinical symptoms. In this study, we established a collagen-induced arthritis (CIA) model and found a large number of M1 macrophages and pyroptosis, which are important sources of proinflammatory cytokines. Punicalagin (PUN) is an active substance extracted from pomegranate peel. We found that it inhibited joint inflammation, cartilage damage and systemic bone destruction in CIA mice. PUN effectively alleviated the high expression of inflammatory cytokines in synovial tissue in vivo . PUN treatment shifted macrophages from the M1 phenotype to the M2 phenotype after stimulation with lipopolysaccharide (LPS) and interferon (IFN)-γ. The expression of inducible nitric oxide synthase (iNOS) and other proinflammatory cytokines released by M1 macrophages was decreased in the PUN treatment group. However, simultaneously, the expression of markers of anti-inflammatory M2 macrophages, such as arginase (Arg)-1 and interleukin (IL)-10, was increased. In addition, PUN treatment attenuated pyroptosis by downregulating the expression of NLRP3 and caspase-1, thereby preventing inflammatory cell death resulting from the release of IL-1β and IL-18. Mechanistically, PUN inhibited the activation of receptor activators of the nuclear factor-κB (NF-κB) signaling pathway, which contributes to M1 polarization and pyroptosis of macrophages. We concluded that PUN ameliorated pathological inflammation by inhibiting M1 phenotype polarization and pyroptosis and has great potential as a therapeutic treatment for human RA.

• Publisher: Science China Press,Springer Nature B.V
• Subject: Animals / Arginase / Arthritis / Arthritis, Experimental - drug therapy / Arthritis, Experimental - immunology / Arthritis, Rheumatoid - drug therapy / Biomedical and Life Sciences / Bone loss / Cartilage / Caspase 1 - physiology / Caspase-1 / Cell death / Cells, Cultured / Collagen / Cytokines / Cytokines - analysis / Down-Regulation / Genotype & phenotype / Health services / Hydrolyzable Tannins - pharmacology / Hydrolyzable Tannins - therapeutic use / IL-1β / Inflammatory diseases / Interferon / Interleukin 18 / Joints (anatomy) / Life Sciences / Lipopolysaccharides / Macrophages / Macrophages - drug effects / Male / Mice / Mice, Inbred DBA / NF-kappa B - physiology / NF-κB protein / Nitric oxide / Nitric-oxide synthase / NLR Family, Pyrin Domain-Containing 3 Protein - antagonists & inhibitors / Phenotypes / Polarization / Pyroptosis / Pyroptosis - drug effects / Research Paper / Rheumatoid arthritis / Signal transduction / Signal Transduction - drug effects / Signal Transduction - physiology