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miR-195 regulates SIRT1-mediated changes in diabetic retinopathy
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miR-195 regulates SIRT1-mediated changes in diabetic retinopathy
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Journal Article
miR-195 regulates SIRT1-mediated changes in diabetic retinopathy
2014
Overview
Aims/hypothesis
Endothelial cell (EC) damage is a key mechanism causing retinal microvascular injury in diabetes. Several microRNAs (miRNAs) have been found to regulate sirtuin 1 (SIRT1, which is involved in regulation of the cell cycle, survival and metabolism) in various tissues and disease states, but no studies have been conducted on the role of miRNA in regulation of SIRT1 in diabetic retinopathy. Here we investigated the effect of miRNA-195 (miR-195), a
SIRT1
-targeting miRNA, on the development of diabetes-induced changes in ECs and retina.
Methods
The level of miR-195 was measured in human retinal and dermal microvascular ECs (HRECs, HMECs) following exposure to 25 mmol/l glucose (high glucose, HG) and 5 mmol/l glucose (normal glucose, NG). SIRT1 and fibronectin levels were examined following transfection with miR-195 mimic or antagomir or forced expression of
SIRT1
. Retinal tissues from diabetic rats were similarly studied following intravitreal injection of an miR-195 antagomir or mimic. In situ hybridisation was used to localise retinal miR-195.
Results
HG caused increased miR-195 levels and decreased
SIRT1
expression (compared with NG) in both HRECs and HMECs. Transfection with miR-195 antagomir and forced expression of
SIRT1
prevented such changes, whereas transfection with miR-195 mimic produced HG-like effects. A luciferase assay confirmed the binding of miR-195 to the 3′ untranslated region of
SIRT1
. miR-195 expression was upregulated in retinas of diabetic rats and intravitreal injection of miR-195 antagomir ameliorated levels of SIRT1.
Conclusions/interpretation
These studies identified a novel mechanism whereby miR-195 regulates SIRT1-mediated tissue damage in diabetic retinopathy.
Publisher
Springer Berlin Heidelberg,Springer,Springer Nature B.V
Subject
/ Aging
/ Animals
/ Biological and medical sciences
/ Diabetes
/ Diabetes Mellitus - metabolism
/ Diabetes. Impaired glucose tolerance
/ Diabetic Retinopathy - metabolism
/ Endocrine pancreas. Apud cells (diseases)
/ Endothelial Cells - metabolism
/ Etiopathogenesis. Screening. Investigations. Target tissue resistance
/ Genes
/ Glucose
/ Humans
/ Male
/ Medicine
/ Rats
/ Retina
