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Nerve growth factor and substance P: expression in a rat model of diabetic bladder
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Nerve growth factor and substance P: expression in a rat model of diabetic bladder
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Journal Article
Nerve growth factor and substance P: expression in a rat model of diabetic bladder
2011
Overview
Objective
To evaluate the gene and protein expression of nerve growth factor (NGF) and substance P (SP) in the bladder 8 weeks after diabetes induction and investigate the pathogenesis of diabetic cystopathy.
Methods
Thirty Wistar rats were divided into three groups: control (
n
= 10), streptozotocin-induced diabetic group (
n
= 10) and
Pygeum africanum
(
P. africanum
) group (
n
= 10; diabetic rats were given
P. africanum
(100 mg/kg/day)). Eight weeks later, the bladders were dissected. We measured the expression of NGF and SP in the bladders using RT–PCR, ELISA and immunohistochemistry.
Results
We found a significantly reduced expression of NGF in the bladders from the diabetic group compared with the control. Immunohistochemical studies showed a statistically significant reduction of SP in the bladders from the diabetic group compared with the control (
P
< 0.05). Expression of NGF was greatly increased in the
P. africanum
group compared with that of the diabetic group. Immunohistochemical studies showed an increased level of SP in the bladders from the
P. africanum
group compared with the control (
P
< 0.05).
Conclusions
Our findings indicated that the decrease in NGF and SP may be a contributory factor in diabetic cystopathy. In addition,
P. africanum
could significantly upregulate the expression of NGF and SP in diabetic rats.
Publisher
Springer Netherlands,Springer Nature B.V
Subject
/ Diabetes Mellitus, Experimental - complications
/ Diabetes Mellitus, Experimental - genetics
/ Diabetes Mellitus, Experimental - metabolism
/ Enzyme-Linked Immunosorbent Assay
/ Male
/ Medicine
/ Nerve Growth Factor - biosynthesis
/ Nerve Growth Factor - genetics
/ Rats
/ Reverse Transcriptase Polymerase Chain Reaction
/ Urinary Bladder Diseases - etiology
/ Urinary Bladder Diseases - genetics
/ Urinary Bladder Diseases - metabolism
/ Urology
