Journal Article

CD14highCD16+ monocytes are the main producers of Interleukin-10 following clinical heart transplantation

Keil, Jana, Chichelnitskiy, Evgeny, Ludwig, Kristina, Wiegmann, Bettina, Ius, Fabio, Rojas, Sebastian V., Falk, Christine S., Haverich, Axel, Knoefel, Ann-Kathrin, Beushausen, Kerstin, Kühne, Jenny F., Ledwoch, Nadine, Warnecke, Gregor, Salman, Jawad, Iske, Jasper, Iordanidis, Susanne

2023

Overview

IntroductionFollowing heart transplantation, a cascade of immunological responses is initiated influencing the clinical outcome and long-term survival of the transplanted patients. The anti-inflammatory cytokine interleukin-10 (IL-10) was shown to be elevated in the blood of heart transplant recipients directly after transplantation but the releasing cell populations and the composition of lymphocyte subsets following transplantation have not been thoroughly studied.MethodsWe identified immune cells by immunophenotyping and analyzed intracellular IL-10 production in peripheral blood mononuclear cells (PBMC) of heart transplanted patients (n= 17) before, directly after and 24h post heart transplantation. The cells were stimulated with lipopolysaccharide or PMA/Ionomycin to enhance cytokine production within leukocytes in vitro .Results and discussionWe demonstrate that intermediate monocytes (CD14highCD16+), but not CD8+ T cells, CD4+ T cells, CD56+ NK cells or CD20+ B cells appeared to be the major IL-10 producers within patients PBMC following heart transplantation. Consequently, the absolute monocyte count and the ratio of intermediate monocytes to classical monocytes (CD14+CD16-) were specifically increased in comparison to pre transplant levels. Hence, this population of monocytes, which has not been in the focus of heart transplantation so far, may be an important modulator of clinical outcome and long-term survival of heart transplant recipients. Alteration of blood-circulating monocytes towards a CD14highCD16+ phenotype could therefore shift the pro-inflammatory immune response towards induction of graft tolerance, and may pave the way for the optimization of immunosuppression.

• Publisher: Frontiers Media SA,Frontiers Media S.A
• Subject: Cardiomyopathy / CD14 antigen / CD16 antigen / CD20 antigen / CD4 antigen / CD56 antigen / CD8 antigen / Chemokines / Cold storage / Cytokines / Flow cytometry / Heart failure / Heart transplantation / Heart transplants / Immune response / Immunological tolerance / Immunology / Immunosuppression / Inflammation / Interleukin 10 / Ionomycin / Ischemia / ischemia-reperfusion injury / Leukocytes / Leukocytes (mononuclear) / Lipopolysaccharides / Lymphocytes / Lymphocytes B / Lymphocytes T / Monocytes / organ transplantation / Patients / Peripheral blood mononuclear cells / Phenotypes / Plasma / Software / Standard of care / T cell receptors