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The positive impact of the NtTAS14-like1 gene on osmotic stress response in Nicotiana tabacum
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The positive impact of the NtTAS14-like1 gene on osmotic stress response in Nicotiana tabacum
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Journal Article
The positive impact of the NtTAS14-like1 gene on osmotic stress response in Nicotiana tabacum
2024
Overview
Key message
NtTAS14-like1
enhances osmotic tolerance through coordinately activating the expression of osmotic- and ABA-related genes.
Osmotic stress is one of the most important limiting factors for tobacco (
Nicotiana tabacum
) growth and development. Dehydrin proteins are widely involved in plant adaptation to osmotic stress, but few of these proteins have been functionally characterized in tobacco. Here, to identify genes required for osmotic stress response in tobacco, an encoding dehydrin protein gene
NtTAS14-like1
was isolated based on RNA sequence data. The expression of
NtTAS14-like1
was obviously induced by mannitol and abscisic acid (ABA) treatments. Knock down of
NtTAS14-like1
expression reduced osmotic tolerance, while overexpression of
NtTAS14-like1
conferred tolerance to osmotic stress in transgenic tobacco plants, as determined by physiological analysis of the relative electrolyte leakage and malonaldehyde accumulation. Further expression analysis by quantitative real-time PCR indicated that NtTAS14-like1 participates in osmotic stress response possibly through coordinately activating osmotic- and ABA-related genes expression, such as
late embryogenesis abundant
(
NtLEA5
),
early responsive to dehydration 10C
(
NtERD10C
)
, calcium-dependent protein kinase 2
(
NtCDPK2
),
ABA-responsive element-binding protein
(
NtAREB
),
ABA-responsive element-binding factor 1
(
NtABF1
),
dehydration-responsive element-binding genes
(
NtDREB2A
),
xanthoxin dehydrogenase/reductase
(
NtABA2
)
, ABA-aldehyde oxidase 3
(
NtAAO3
)
, 9-cis-epoxycarotenoid dioxygenase
(
NtNCED3
). Together, this study will facilitate to improve our understandings of molecular and functional properties of plant TAS14 proteins and to improve genetic evidence on the involvement of the
NtTAS14-like1
in osmotic stress response of tobacco.
Publisher
Springer Berlin Heidelberg,Springer Nature B.V
Subject
9-cis-epoxycarotenoid dioxygenase
/ Abscisic Acid - pharmacology
/ Biomedical and Life Sciences
/ Calcium
/ Dehydrin
/ Gene Expression Regulation, Plant - genetics
/ Genes
/ genetically modified organisms
/ Kinases
/ Mannitol
/ Osmotic Pressure - physiology
/ Plants, Genetically Modified - genetics
/ Plants, Genetically Modified - metabolism
/ Proteins
/ quantitative polymerase chain reaction
/ Stress, Physiological - genetics
/ Tobacco
