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Nitric oxide and hydrogen peroxide increase glucose-6-phosphate dehydrogenase activities and expression upon drought stress in soybean roots
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Nitric oxide and hydrogen peroxide increase glucose-6-phosphate dehydrogenase activities and expression upon drought stress in soybean roots
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Journal Article
Nitric oxide and hydrogen peroxide increase glucose-6-phosphate dehydrogenase activities and expression upon drought stress in soybean roots
2020
Overview
Key message
Changes in glucose-6-phosphate dehydrogenase (G6PD) isoforms activities and expression were investigated in soybean roots under drought, suggesting that cytosolic G6PD plays a main role by regulating H
2
O
2
signal and redox homeostasis.
G6PD acts a vital role in plant growth, development and stress adaptation. Drought (PEG6000 treatment) could markedly increase the enzymatic activities of cytosolic G6PD
(
Cyt-G6PD
)
and compartmented G6PD (mainly plastidic P2-G6PD) in soybean roots. Application of G6PD inhibitor upon drought condition dramatically decreased the intracellular NADPH and reduced glutathione levels in soybean roots. Nitric oxide (NO) and hydrogen peroxide (H
2
O
2
) participated in the regulation of Cyt-G6PD and P2-G6PD enzymatic activities under drought stress. Diphenylene iodonium (DPI), an inhibitor of NADPH oxidase, abolished the drought-induced accumulation of H
2
O
2
. The exogenous application of H
2
O
2
and its production inhibitor (DPI) could stimulate and inhibit the NO accumulation, respectively, but not vice versa. qRT-PCR analysis confirmed that NO, as the downstream signal of H
2
O
2
, positively regulated the transcription of genes encoding Cyt-G6PD (
GPD5
,
G6PD6
,
G6PD7
) under drought stress in soybean roots. Comparatively, NO and H
2
O
2
signals negatively regulated the gene expression of compartmented G6PD (
GPD1
,
G6PD2
,
G6PD4
), indicating that a post-transcriptional mechanism was involved in compartmented G6PD regulation. Taken together, the high Cyt-G6PD activity is essential for maintaining redox homeostasis upon drought condition in soybean roots, and the H
2
O
2
-dependent NO cascade signal is differently involved in Cyt-G6PD and compartmented G6PD regulation.
Publisher
Springer Berlin Heidelberg,Springer Nature B.V
Subject
/ Adaptation, Physiological - drug effects
/ Adaptation, Physiological - genetics
/ Biomedical and Life Sciences
/ Drought
/ Droughts
/ genes
/ Glucose
/ Glucose 6 phosphate dehydrogenase
/ Glucosephosphate dehydrogenase
/ Glucosephosphate Dehydrogenase - antagonists & inhibitors
/ Glucosephosphate Dehydrogenase - biosynthesis
/ Glucosephosphate Dehydrogenase - genetics
/ Glucosephosphate Dehydrogenase - metabolism
/ Glycerol-3-phosphate dehydrogenase
/ Hydrogen Peroxide - metabolism
/ Isoforms
/ NAD(P)H oxidase (H2O2-forming)
/ NADP-glucose-6-phosphate dehydrogenase
/ Onium Compounds - pharmacology
/ quantitative polymerase chain reaction
/ Reactive Oxygen Species - metabolism
/ reverse transcriptase polymerase chain reaction
/ Roots
/ Soybeans
/ Stress, Physiological - genetics
