Journal Article

MiR-320a upregulation contributes to the effectiveness of pemetrexed by inhibiting the growth and invasion of human lung cancer cell line (Calu-6)

Arpanaei, Ayyoob, Esmaeili Gouvarchin Ghaleh, Hadi, Ghorbani Alvanegh, Akbar, Mohammad Ganji, Shahla

2024

Overview

Background Lung cancer is a common and deadly disease. Chemotherapy is the most common treatment, which inhibits cancer cell growth. Pemetrexed (PMX) is often used with other drugs. Environmental stress can affect regulatory non-coding RNAs such as MicroRNAs that modify gene expression. This study investigates the effect of PMX on the hsa-miR-320a-3p expression in the Calu-6 lung cancer cell line. Methods and result Calu-6 cells were cultured in an incubator with 37 °C, 5% CO2, and 98% humidity. The MTT test was performed to determine the concentration of PMX required to inhibit 50% of cell growth. To examine growth inhibition and apoptosis, release of lactate dehydrogenase (LDH), cell assays and caspase 3 and 7 enzyme activity were used. Finally, molecular studies were conducted to compare the expression of hsa-miR-320a-3p and genes including VDAC1, DHFR, STAT3, BAX and BCL2 before and after therapy. Results According to a study, it has been observed that PMX therapy significantly increases LDH release after 24 h. The study found that PMX's IC 50 on Calu-6 is 8.870 µM. In addition, the treated sample showed higher expression of hsa-miR-320a-3p and BAX , while the expression of VDAC1, STAT3, DHFR and BCL2 decreased compared to the control sample. Conclusion According to the findings of the current research, hsa-miR-320a-3p seems to have the potential to play an important role in the development of novel approaches to the treatment of lung cancer.

• Publisher: Springer Netherlands,Springer Nature B.V
• Subject: Animal Anatomy / Animal Biochemistry / Apoptosis / BAX protein / Bcl-2 protein / bcl-2-Associated X Protein - genetics / Biomedical and Life Sciences / Cancer therapies / Carbon dioxide / Caspase-3 / Cell growth / Cell Line / cell lines / Chemotherapy / Chromosome 3 / Dihydrofolate reductase / drug therapy / Environmental stress / Enzymatic activity / enzyme activity / Gene expression / growth retardation / Histology / Humans / humidity / L-Lactate dehydrogenase / lactate dehydrogenase / Life Sciences / Lung cancer / lung neoplasms / Lung Neoplasms - drug therapy / Lung Neoplasms - genetics / microRNA / MicroRNAs - genetics / miRNA / Morphology / neoplasm cells / Original Article / Pemetrexed - pharmacology / Stat3 protein / Up-Regulation - genetics