Asset Details
Do you wish to reserve the book?
Development of an immortalized porcine intramuscular pre-adipocyte cell line to facilitate adipogenic investigation in vitro
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Development of an immortalized porcine intramuscular pre-adipocyte cell line to facilitate adipogenic investigation in vitro
Do you wish to request the book?
Development of an immortalized porcine intramuscular pre-adipocyte cell line to facilitate adipogenic investigation in vitro
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Development of an immortalized porcine intramuscular pre-adipocyte cell line to facilitate adipogenic investigation in vitro
2025
Overview
Intramuscular fat (IMF) deposition critically influences pork quality, necessitating reliable in vitro models to investigate its regulatory mechanisms. However, developing cost-effective porcine intramuscular pre-adipocyte (IMPA) seed cells capable of sustained proliferation and complete adipogenic differentiation remains technically challenging. Here, we established an immortalized porcine IMPA cell line through lentiviral-mediated transduction of simian virus 40 large T antigen (SV40T). This cell line retained differentiation capacity for over 25 passages, addressing limitations in primary cell lifespan. Functional evaluations demonstrated robust proliferative activity via CCK-8 assays, sustained telomerase activity, and minimal apoptosis, confirming stable growth characteristics during long-term culture. Furthermore, adipogenic induction assays revealed preserved differentiation potential, evidenced by lipid droplet accumulation and adipogenic marker expression. Collectively, this immortalized porcine IMPA cell line provides a valuable and reproducible model for elucidating molecular mechanisms underlying IMF deposition, offering significant utility for agricultural and biomedical research.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Cells
/ Ethylenediaminetetraacetic acid
/ Hogs
/ IMF
/ Intramuscular pre-adipocytes
/ Meat
/ Medicine
/ Pig
/ Pork
/ Quality
/ SV40T
/ Swine
/ Veterinary Medicine/Veterinary Science
/ Zoology
