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Analysis of the urinary glucose-15N, 15N-ureide content in the study of the lactose-15N, 15N-ureide metabolism in healthy humans
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Analysis of the urinary glucose-15N, 15N-ureide content in the study of the lactose-15N, 15N-ureide metabolism in healthy humans
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Journal Article
Analysis of the urinary glucose-15N, 15N-ureide content in the study of the lactose-15N, 15N-ureide metabolism in healthy humans
2011
Overview
Background/Objectives:
Lactose-[
15
N,
15
N]-ureide is used to study the fate of the colonic urea-nitrogen metabolism. During the passage through the gastrointestinal tract, lactose ureide is hydrolysed to glucose ureide, which is absorbed to a limited extent from the small intestine and is excreted urinarily. In the present study, a procedure has been developed to quantify the urinary excretion of glucose-[
15
N,
15
N]-ureide. In addition, urine and faecal samples obtained during a dietary intervention study with the prebiotic lactulose were retrospectively analysed.
Subjects/Methods:
The glucose ureide and lactose ureide content was measured by GC–MS in 19 healthy volunteers. After consumption of a standard test meal containing 75 mg lactose-[
15
N,
15
N]-ureide, six healthy volunteers performed a fractionated 24 h urine collection to investigate the urinary excretion of glucose-[
15
N,
15
N]-ureide. In 13 volunteers, the effect of lactulose administration on the urinary excretion of glucose-[
15
N,
15
N]-ureide was analysed.
Results:
The urinary excretion of glucose-[
15
N,
15
N]-ureide reached its maximum level in the 3–6 h urine collection and decreased in the 6–9 h urine. The label was still detectable in the 9–24 h urine collection. The cumulative excretion of
15
N-labelled glucose ureide after 24 h amounted 12.91%. No significant differences in glucose-[
15
N,
15
N]-ureide excretion were found in either of the urine fractions after administration of lactulose, compared with baseline. In none of the urine samples lactose-[
15
N,
15
N]-ureide was detected.
Conclusions:
In conclusion, the results obtained in the present study indicated that the percentage dose glucose-[
15
N,
15
N]-ureide recovered in urine is rather constant and not influenced by the presence of lactulose.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Adult
/ Biological and medical sciences
/ Diet
/ Feces
/ Female
/ Fundamental and applied biological sciences. Psychology
/ Gas Chromatography-Mass Spectrometry
/ Glucose
/ Glucose - analogs & derivatives
/ Humans
/ Lactose
/ Lactulose - administration & dosage
/ Male
/ Medicine
/ Nitrogen
/ Urea
/ Urea - analogs & derivatives
/ Urine
/ Vertebrates: anatomy and physiology, studies on body, several organs or systems
