Journal Article

Priming Adipose Stem Cells with Tumor Necrosis Factor-Alpha Preconditioning Potentiates Their Exosome Efficacy for Bone Regeneration

Zreiqat, Hala, Roohani-Esfahani, Seyediman, Lu, ZuFu, Dunstan, Colin, Chen, YongJuan

2017

Overview

Mesenchymal stem cells (MSCs) have been widely used for tissue repair and regeneration. However, the inherent drawbacks, including limited cell survival after cell transplantation, have hindered direct MSC transplantation for tissue repair and regeneration. The aim of this study was to investigate if exosomes isolated from MSCs can promote the proliferation and differentiation of human primary osteoblastic cells (HOBs) and be potentially used for bone tissue regeneration. We showed that adipose tissue-derived MSC (ASC)-derived exosomes (ASC-EXO) were able to promote the proliferation and osteogenic differentiation in HOBs; and the trophic effects of ASC-EXO on HOBs were further harnessed when ASCs were preconditioned with tumor necrosis factor-alpha (TNF-α) for 3 days, which mimics the acute inflammatory phase upon bone injury. In addition, we showed that Wnt-3a content was elevated in ASC-EXO when ASCs were preconditioned by TNF-α, and inhibiting Wnt signaling decreased the osteogenic gene expression levels in HOBs which were cultured in TNF-α preconditioned ASCs conditioned medium. In conclusion, it was demonstrated that ASC-EXO, especially primed by TNF-α preconditioning on ASCs, offer a promising approach to replace direct stem cell transplantation for bone repair and regeneration.

• Publisher: Mary Ann Liebert, Inc,SAGE Publications
• Subject: Adipose tissue / Adipose Tissue - cytology / Biomarkers / Biomedical materials / Bone growth / Bone healing / Bone marrow / Bone Regeneration - drug effects / Cardiomyocytes / Cell Differentiation - drug effects / Cell Movement - drug effects / Cell proliferation / Cell Proliferation - drug effects / Cell survival / Culture Media, Conditioned - pharmacology / Endocytosis - drug effects / Engineering research / Exosomes / Exosomes - drug effects / Exosomes - metabolism / Gene expression / Growth factors / Humans / Hypoxia / Inflammation / Medicine / Mesenchyme / Necrosis / Osteoblastogenesis / Osteoblasts / Osteoblasts - cytology / Osteoblasts - drug effects / Osteoblasts - metabolism / Osteogenesis - drug effects / Regeneration / Special Focus Articles / Stem cell transplantation / Stem cells / Stem Cells - cytology / Stem Cells - drug effects / Stem Cells - metabolism / Studies / Tissue engineering / Transplantation / Transplants & implants / Tumor Necrosis Factor-alpha - pharmacology / Tumor necrosis factor-TNF / Tumor necrosis factor-α / Wnt protein / Wnt3A Protein - metabolism / Wound healing