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Characterization of AT2 Receptor Expression in NIH 3T3 Fibroblasts
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Characterization of AT2 Receptor Expression in NIH 3T3 Fibroblasts
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Characterization of AT2 Receptor Expression in NIH 3T3 Fibroblasts
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Journal Article
Characterization of AT2 Receptor Expression in NIH 3T3 Fibroblasts
1999
Overview
1. A high expression of angiotensin II receptors and of angiotensin-converting enzyme (ACE) activity was detected in confluent NIH 3T3 fibroblasts.2. Characterization with selective ligands, dithiothreitol, and GTPγS, indicated that only the AT2 subtype was expressed.3. AT2 receptors and ACE expression were strictly dependent on the cell density and growth phase of the cells, with AT2 receptors being expressed earlier than ACE. In contrast, high expression of AT2 receptors irrespective of their growth state was observed in NIH 3T3 cells lacking contact inhibition upon neoplastic transformation with ras.4. Our results imply a possible relation of AT2 receptors to cell growth and cell–cell contact.
Publisher
Springer Nature B.V
