Asset Details
Do you wish to reserve the book?
Antifolate Resistance in Plasmodium falciparum: Multiple Origins and Identification of Novel dhfr Alleles
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Antifolate Resistance in Plasmodium falciparum: Multiple Origins and Identification of Novel dhfr Alleles
Do you wish to request the book?
Antifolate Resistance in Plasmodium falciparum: Multiple Origins and Identification of Novel dhfr Alleles
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Antifolate Resistance in Plasmodium falciparum: Multiple Origins and Identification of Novel dhfr Alleles
2006
Overview
BackgroundSulfadoxine-pyrimethamine has been widely used as first-line therapy for uncomplicated malaria throughout sub-Saharan Africa. Recent studies conducted in Asia and Africa suggest the triple-mutant dhfr genotype (51I/59R/108N) may have been generated as a single event in Southeast Asia, with subsequent spread of the single lineage to the African continent, but this hypothesis needs further validation MethodsDirect sequencing of polymerase chain reaction (PCR) products, pyrosequencing, and cloning of PCR products were utilized to identify mutations in dhfr. To investigate the evolutionary history of dhfr alleles, we assayed microsatellite loci flanking dhfr along chromosome 4 ResultsA total of 15 of 479 samples from western Kenya showed the presence of I164L, in 5 different genotypes. We document C50R in 2 of our samples. Using microsatellite markers, we show 2 haplotypes for both the 51I/108N/164L and 51I/59R/108N/164L genotypes. Our results also show multiple lineages for the triple-mutant dhfr genotype in Africa ConclusionsThese findings highlight the importance of local characterization of alleles before molecular surveillance of drug-resistant alleles is considered in different endemic settings and populations
Publisher
The University of Chicago Press,University of Chicago Press,Oxford University Press
Subject
/ Animals
/ Antimalarials - pharmacology
/ Codons
/ Folic Acid Antagonists - pharmacology
/ Genotype
/ HIV Infections - complications
/ Humans
/ Malaria
/ Malaria, Falciparum - parasitology
/ Microsatellite Repeats - genetics
/ Mutation
/ Plasmodium falciparum - drug effects
/ Plasmodium falciparum - enzymology
/ Plasmodium falciparum - genetics
