Journal Article

Protective effect of oxysophoridine on cerebral ischemia/reperfusion injury in mice

Hongbo Wang Yuxiang Li Ning Jiang Xiaoping Chen Yi Zhang Kuai Zhang Tengfei Wang Yinju Hao Lin Ma Chengjun Zhao Yanrong Wang Tao Sun Jianqiang Yu

2013

Overview

Oxysophoridine, a new alkaloid extracted from Sophora alopecuroides L., has been shown to have a protective effect against ischemic brain damage. In this study, a focal cerebral ischemia/reperfusion injury model was established using middle cerebral artery occlusion in mice. Both 62.5, 125, and 250 mg/kg oxysophoridine, via intraperitoneal injection, and 6 mg/kg nimodipine, via intragastric administration, were administered daily for 7 days before modeling. After 24 hours of reperfusion, mice were tested for neurological deficit, cerebral infarct size was assessed and brain tissue was collected. Results showed that oxysophoridine at 125, 250 mg/kg and 6 mg/kg nimodipine could reduce neurological deficit scores, cerebral infarct size and brain water content in mice. These results provided evidence that oxysophoridine plays a protective role in cerebral ischemia/reperfusion injury. In addition, oxysophoridine at 62.5, 125, and 250 mg/kg and 6 mg/kg nimodipine increased adenosine-triphosphate content, and decreased malondialdehyde and nitric oxide content. These compounds enhanced the activities of glutathione-peroxidase, superoxide dismutase, catalase, and lactate dehydrogenase, and decreased the activity of nitric oxide synthase Protein and mRNA expression levels of N-methyI-D-aspartate receptor subunit NR1 were markedly inhibited in the presence of 250 mg/kg oxysophoridine and 6 mg/kg nimodipine. Our experimental findings indicated that oxysophoridine has a neuroprotective effect against cerebral ischemia/reperfusion injury in mice, and that the effect may be due to its ability to inhibit oxidative stress and expression of the N-methyI-D-aspartate receptor subunit NR1.

• Publisher: Medknow Publications and Media Pvt. Ltd,Medknow Publications & Media Pvt. Ltd,Shanghai Pudong New Area Gongli Hospital, Shanghai 200135, China%Shanghai Pudong New Area Gongli Hospital, Shanghai 200135, China%Ningxia Key Laboratory of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Yinchuan 750004, Ningxia Hui Autonomous Region, China%School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China%Key Laboratory of Reproduction and Genetics of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China%Department of Pharmacology, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China,Collaborative Innovation Center of Ningxia Hui Autonomous Region for Medicines, Yinchuan 750004, Ningxia Hui Autonomous Region, China,Department of Pharmacology, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China%College of Nursing, Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China,Medknow Publications & Media Pvt Ltd
• Subject: Adenosine / Alkaloids / Antioxidants / Beans / Brain damage / Care and treatment / Cerebral ischemia / Coronary vessels / Cytotoxicity / D-天冬氨酸 / Dehydrogenases / Drug dosages / Edema / Enzymes / Health aspects / Herbal medicine / Ischemia / Legumes / Materia medica, Vegetable / Medicinal plants / Metabolism / Mimosaceae / Nitric oxide / Oxidative stress / Plant extracts / Protein expression / Proteins / Reperfusion injury / Research and Report : Traditional Chinese Medicine and Neural Regeneration / Studies / Traumatic brain injury / Veins & arteries / 一氧化氮含量 / 槐 / 生物碱 / 神经保护作用 / 缺血;再灌注损伤 / 缺血性脑损伤 / 谷胱甘肽过氧化物酶