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Editorial: Methods and applications of CRISPR technology in plant sciences, 2022
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Editorial: Methods and applications of CRISPR technology in plant sciences, 2022
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Editorial: Methods and applications of CRISPR technology in plant sciences, 2022
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Journal Article
Editorial: Methods and applications of CRISPR technology in plant sciences, 2022
2023
Overview
Efficiency of the CRISPR/Cas approach depends on several factors including but not limited to: (i) the type of Cas nuclease optimized to plant codons (ii) the presence and number of the NLS that can affect the Cas9 delivery to the cell nuclei, (iii) source and nature of promoters used to drive the expression of Cas genes, and (iv) an appropriate choice of target site and construction of gRNAs which can increase the mutation frequency. [...]the editing success is contingent on the type of transgene delivery to the host/plant genome. The authors also address the bioengineering role of fatty acid biosynthesis in enhancing the commercial production of vegetable oil for human consumption.
