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Characterizing White Matter Damage in Rat Spinal Cord with Quantitative MRI and Histology
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Characterizing White Matter Damage in Rat Spinal Cord with Quantitative MRI and Histology
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Journal Article
Characterizing White Matter Damage in Rat Spinal Cord with Quantitative MRI and Histology
2008
Overview
Diffusion tensor imaging (DTI) and quantitative T2 magnetic resonance imaging (MRI) were used to characterize ex vivo the white matter damage at 3 and 8 weeks following dorsal column transection (DC Tx) injury at the cervical level C5 of rat spinal cords. Luxol Fast Blue (LFB) and myelin basic protein (MBP) staining was used to assess myelin damage, and neurofilament-H in combination with neuron specific β-III-tubulin (NF/Tub) staining was used to assess axonal damage. Average values of myelin water fraction (MWF), fractional anisotropy (FA), longitudinal diffusivity (Dlong), transverse diffusivity (Dtrans), and average diffusivity (Dave) were calculated in the fasciculus gracilis, fasciculus cuneatus, and the dorsal corticospinal tract (CST) 5 mm cranial, as well as 5 and 10 mm caudal to injury and correlated with histology. These tracts were selected as these contain bundles of parallel ascending and descending axons in very circumscribed areas with little intermingling of other axonal populations. Axonal and myelin degeneration occur cranial to injury in the funiculus gracilis and caudal to injury in the CST. Both MWF and Dtrans showed significant correlation with LFB staining at 3 weeks (0.64 and −0.49, respectively) and 8 weeks post-injury (0.88 and −0.71, respectively). Both Dlong and FA correlated significantly with NF/Tub staining at 3 weeks post-injury (0.78 and 0.64, respectively), while only Dlong displayed significant correlation 8 weeks post-injury (0.58 and 0.33, respectively). This study demonstrates that quantitative MRI can accurately characterize white matter damage in DC Tx model of injury in rat spinal cord.
Publisher
SAGE Publications,Mary Ann Liebert, Inc
Subject
/ Diffusion Magnetic Resonance Imaging - methods
/ Male
/ Myelin Basic Protein - analysis
/ Myelin Basic Protein - metabolism
/ Nerve Fibers, Myelinated - pathology
/ Neural Pathways - physiopathology
/ Neurofilament Proteins - analysis
/ Neurofilament Proteins - metabolism
/ NMR
/ Rats
/ Rodents
/ Spinal Cord - physiopathology
/ Spinal Cord Injuries - pathology
/ Spinal Cord Injuries - physiopathology
/ Staining and Labeling - methods
/ Wallerian Degeneration - etiology
