Journal Article

Circulation of human papillomavirus (HPV) genotypes in women from Córdoba, Argentina, with squamous intraepithelial lesions

Kiguen, Ana Ximena, Venezuela, Raul Fernando, Cuffini, Cecilia Gabriela, Frutos, Maria Celia

2012

Overview

Human papillomavirus (HPV) can induce a wide spectrum of squamous intraepithelial lesions (SIL) of varying severity. The aim of the present study was to establish the frequency of HPV infection and identify the genotypes circulating in women from Córdoba, Argentina, in relation to age and cytology. A total of 186 women, between 18 and 65 years old, with antecedents of SIL, underwent a pelvic examination and had cervical cells collected for cytology and HPV DNA detection. Ninety-six samples (51.6%) were positive for HPV detection, and sixty-three (65.6%) of them showed the presence of at least one HR-HPV. Low- and high-grade SIL showed significant association in patients younger than 35 years of age. We found 18 different genotypes, with a greater presence of HR-HPV. Genotypes 16 and 6 were the most frequent. Seven (7.3%) multiple infections, 85.7% of which had at least one HR-HPV, were detected. The detection of a large number of different HPV genotypes is a warning sign. It is thus necessary to strengthen the monitoring of the circulation of high-risk genotypes, currently less prevalent in intraepithelial lesions, as a control measure for the possible impact of the implementation of vaccines against genotypes 16 and 18. El papilomavirus humano (human papilloma, HPV) induce un amplio espectro de lesiones intraepiteliales escamosas (SIL) de variada severidad. Objetivo: conocer la frecuencia de infección por HPV y determinar los genotipos circulantes en mujeres de la ciudad de Córdoba, Argentina, en relación con la edad y la citología. Se realizó citología y detección de ADN-HPV en células cervicales de 186 mujeres de 18 a 65 años con antecedentes de SIL. Noventa y seis (51.6%) fueron positivas para la detección del HPV, de las cuales, en 63 (65.6%) se detectó la presencia de al menos, un HPV de Alto Riesgo (HR-HPV). Las SIL de alto grado (HSIL) y de bajo grado (LSIL) se asociaron a pacientes menores de 35 años. Se hallaron 18 genotipos diferentes, con mayor presencia de HR-HPV. HPV 16 y 6 fueron más frecuentes y se detectaron 7 (7.3%) infecciones múltiples, 85.7% de éstas presentaron al menos un HR-HPV. La detección de un alto número de diferentes genotipos es una señal de alerta. Por tanto, es necesario fortalecer la vigilancia de los HR-HPV, actualmente menos frecuentes en las SIL, como medida de control del impacto que tendrá la implementación de las vacunas contra HPV 16 y 18.

• Publisher: Instituto de Medicina Tropical de Sao Paulo,Instituto de Medicina Tropical,Universidade de São Paulo (USP)
• Subject: Adolescent / Adult / Aged / Argentina - epidemiology / Cervical Intraepithelial Neoplasia - epidemiology / Cervical Intraepithelial Neoplasia - virology / Cervix / DNA, Viral - analysis / Female / Genotype / Genotypes, Argentina / HPV / Humans / Middle Aged / Papillomaviridae - genetics / Papillomaviridae - isolation & purification / Papillomavirus Infections - epidemiology / Papillomavirus Infections - virology / Squamous intraepithelial lesions / TROPICAL MEDICINE / Uterine Cervical Dysplasia - epidemiology / Uterine Cervical Dysplasia - virology / Uterine Cervical Neoplasms - epidemiology / Uterine Cervical Neoplasms - virology / Vaginal Smears / Young Adult