Journal Article

Ambient Particulate Matter Induces In Vitro Toxicity to Intestinal Epithelial Cells without Exacerbating Acute Colitis Induced by Dextran Sodium Sulfate or 2,4,6-Trinitrobenzenesulfonic Acid

Koletic, Carolina, Navab, Mohamad, Xanthou, Georgina, Araujo, Jesus A., Tohidi, Ramin, Hsiai, Tzung, Jacobs, Jonathan P., Chang, Candace, Liang, Fengting, Ereso, Jason, Sedighian, Farzaneh, Gupta, Rajat, Arias-Jayo, Nerea, Zhou, Yi, Louie, Allen, Yang, Julianne Ching, Altuwayjiri, Abdulmalik, Reddy, Srinivasa Tadiparthi, Lagishetty, Venu, Sioutas, Constantinos

2024

Overview

Inflammatory bowel disease (IBD) is an immunologically complex disorder involving genetic, microbial, and environmental risk factors. Its global burden has continued to rise since industrialization, with epidemiological studies suggesting that ambient particulate matter (PM) in air pollution could be a contributing factor. Prior animal studies have shown that oral PM10 exposure promotes intestinal inflammation in a genetic IBD model and that PM2.5 inhalation exposure can increase intestinal levels of pro-inflammatory cytokines. PM10 and PM2.5 include ultrafine particles (UFP), which have an aerodynamic diameter of <0.10 μm and biophysical and biochemical properties that promote toxicity. UFP inhalation, however, has not been previously studied in the context of murine models of IBD. Here, we demonstrated that ambient PM is toxic to cultured Caco-2 intestinal epithelial cells and examined whether UFP inhalation affected acute colitis induced by dextran sodium sulfate and 2,4,6-trinitrobenzenesulfonic acid. C57BL/6J mice were exposed to filtered air (FA) or various types of ambient PM reaerosolized in the ultrafine size range at ~300 μg/m3, 6 h/day, 3–5 days/week, starting 7–10 days before disease induction. No differences in weight change, clinical disease activity, or histology were observed between the PM and FA-exposed groups. In conclusion, UFP inhalation exposure did not exacerbate intestinal inflammation in acute, chemically-induced colitis models.

• Publisher: MDPI AG
• Subject: Air pollution / Animals / Caco-2 Cells / Colitis / Colitis - chemically induced / Colitis - pathology / Colon / Crohn's disease / Cytokines / Dextran / Dextran Sulfate - toxicity / Disease Models, Animal / Epithelial Cells - drug effects / Epithelial Cells - metabolism / Epithelial Cells - pathology / Gastrointestinal diseases / Gastrointestinal system / Humans / Inflammation / Inflammatory bowel disease / Intestinal Mucosa - drug effects / Intestinal Mucosa - metabolism / Intestinal Mucosa - pathology / Male / Mice / Mice, Inbred C57BL / Nitrogen dioxide / Outdoor air quality / Particle Size / Particulate Matter - toxicity / Permeability / Sulfates / Toxicity / Trinitrobenzenesulfonic Acid - adverse effects / Trinitrobenzenesulfonic Acid - toxicity