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Comparative analysis of MAIPA assay and PakAuto assay for the diagnosis of primary immune thrombocytopenia

by Chen, Chaolin , Lai, Jianxi , Tuo, Rui , Su, Hao , Chen, Lixuan , Ye, Limei , Xiao, Yang , Ouyang, Shuting , Feng, Ying

in Adolescent / Adult / Aged / Antibodies / Anticoagulants / Autoantibodies - blood / Blood / Blood platelets / Blood Platelets - immunology / Bone marrow / Comparative analysis / Diagnosis / Enzymes / Female / Flow cytometry / Glycoproteins / Hematology / Humans / Idiopathic thrombocytopenic purpura / Immunoassay - methods / Internal Medicine / Laboratories / Leukemia / Male / Medical research / Medicine / Medicine & Public Health / Middle Aged / Oncology / Platelets / Purpura, Thrombocytopenic, Idiopathic - diagnosis / Purpura, Thrombocytopenic, Idiopathic - immunology / Sensitivity and Specificity / Thrombocytopenia / Young Adult

2025

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Comparative analysis of MAIPA assay and PakAuto assay for the diagnosis of primary immune thrombocytopenia

by Chen, Chaolin , Lai, Jianxi , Tuo, Rui , Su, Hao , Chen, Lixuan , Ye, Limei , Xiao, Yang , Ouyang, Shuting , Feng, Ying

2025

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Comparative analysis of MAIPA assay and PakAuto assay for the diagnosis of primary immune thrombocytopenia

by Chen, Chaolin , Lai, Jianxi , Tuo, Rui , Su, Hao , Chen, Lixuan , Ye, Limei , Xiao, Yang , Ouyang, Shuting , Feng, Ying

2025

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Journal Article

Comparative analysis of MAIPA assay and PakAuto assay for the diagnosis of primary immune thrombocytopenia

Chen, Chaolin,

Lai, Jianxi,

Tuo, Rui,

Su, Hao,

Chen, Lixuan,

Ye, Limei,

Xiao, Yang,

Ouyang, Shuting,

Feng, Ying

2025

Overview

The detection of platelet-specific antibodies plays an important role in the clinical diagnosis of primary immune thrombocytopenia (ITP). MAIPA assay and PakAuto assay are the two main methods for this purpose. However, a comprehensive comparative evaluation of these techniques in clinical practice remains lacking. In this study, we collected 48 patients with thrombocytopenia. MAIPA and PakAuto are used to examine platelet-specific antibodies synchronously. Based on methodologies and analysis of experimental results, we conclude that PakAuto assay with a high sensitivity and accuracy can offer higher throughput and broader human platelet antibodies detection capabilities, making it more suitable for screening.

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Publisher

Springer International Publishing,Springer Nature B.V

Subject

Adolescent

/ Adult

/ Aged

/ Antibodies

/ Anticoagulants

/ Autoantibodies - blood

/ Blood