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Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2
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Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2
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Journal Article
Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2
2020
Overview
We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcription PCR and Sanger sequencing. These protocols enable sensitive virus sequencing in different laboratory environments.
Publisher
U.S. National Center for Infectious Diseases,Centers for Disease Control and Prevention
Subject
/ Coronavirus Infections - virology
/ COVID-19
/ Design
/ Dispatch
/ Genomes
/ Genomics
/ Multiplex Polymerase Chain Reaction
/ Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2
/ Sequence Analysis, RNA - methods
/ Severe acute respiratory syndrome
/ Severe acute respiratory syndrome coronavirus 2
/ viruses
