Asset Details
Do you wish to reserve the book?
Characterization of AT-rich microsatellites in common bean (Phaseolus vulgaris L.)
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Characterization of AT-rich microsatellites in common bean (Phaseolus vulgaris L.)
Do you wish to request the book?
Characterization of AT-rich microsatellites in common bean (Phaseolus vulgaris L.)
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Characterization of AT-rich microsatellites in common bean (Phaseolus vulgaris L.)
2008
Overview
Polymorphism of microsatellite markers is often associated with the simple sequence repeat motif targeted. AT-rich microsatellites tend to be highly variable and this appears to be notable, especially in legume genomes. To analyze the value of AT-rich microsatellites for common bean (
Phaseolus vulgaris
L.), we developed a total of 85 new microsatellite markers, 74 of which targeted ATA or other AT-rich motif loci and 11 of which were made for GA, CA or CAC motif loci. We evaluated the loci for the level of allelic diversity in comparison to previously characterized microsatellites using a panel of 18 standard genotypes and genetically mapped any loci polymorphic in the DOR364 × G19833 population. The majority of the microsatellites produced single bands and detected single loci, however, 15 of the AT-rich microsatellites produced multiple or double banding patterns; while only one of the GA or CA-rich microsatellites did. The polymorphism information content (PIC) values averaged 0.892 and 0.600 for the AT and ATA motif microsatellites, respectively, but only 0.140 for the CA-rich microsatellites. GA microsatellites, which had a large average number of repeats, had high to intermediate PIC, averaging 0.706. A total of 45 loci could be genetically mapped and distribution of the loci across the genome was skewed towards non-distal locations with a greater prevalence of loci on linkage groups b02, b09 and b11. AT-rich microsatellites were found to be a useful source of polymorphic markers for mapping and diversity assessment in common bean that appears to uncover higher diversity than other types of simple sequence repeat markers.
Publisher
Springer-Verlag,Springer,Springer Nature B.V
Subject
/ Alleles
/ Beans
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Classical genetics, quantitative genetics, hybrids
/ Cloning
/ Crops
/ Fundamental and applied biological sciences. Psychology
/ genetics
/ Genetics of eukaryotes. Biological and molecular evolution
/ genome
/ Genomes
/ genotype
/ Legumes
/ loci
/ Plant Breeding/Biotechnology
/ Soybeans
/ Vegetals
We currently cannot retrieve any items related to this title. Kindly check back at a later time.