Asset Details
Do you wish to reserve the book?
Insecticidal fern protein Tma12 is possibly a lytic polysaccharide monooxygenase
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Insecticidal fern protein Tma12 is possibly a lytic polysaccharide monooxygenase
Do you wish to request the book?
Insecticidal fern protein Tma12 is possibly a lytic polysaccharide monooxygenase
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Insecticidal fern protein Tma12 is possibly a lytic polysaccharide monooxygenase
2019
Overview
Tma12, isolated from the fern Tectaria macrodonta, is a next-generation insecticidal protein. Transgenic cotton expressing Tma12 exhibits resistance against whitefly and viral diseases. Beside its insecticidal property, the structure and function of Tma12 are unknown. This limits understanding of the insecticidal mechanism of the protein and targeted improvement in its efficacy. Here we report the amino acid sequence analysis and the crystal structure of Tma12, suggesting that it is possibly a lytic polysaccharide monooxygenase (LPMO) of the AA10 family. Amino acid sequence of Tma12 shows 45% identity with a cellulolytic LPMO of Streptomyces coelicolor. The crystal structure of Tma12, obtained at 2.2 Å resolution, possesses all the major structural characteristics of AA10 LPMOs. A H₂O₂-based enzymatic assay also supports this finding. It is the first report of the occurrence of LPMO-like protein in a plant. The two facts that Tma12 possesses insecticidal activity and shows structural similarity with LPMOs collectively advocate exploration of microbial LPMOs for insecticidal potential.
Publisher
Springer Science + Business Media,Springer Berlin Heidelberg,Springer Nature B.V
Subject
/ Animals
/ Biomedical and Life Sciences
/ Cotton
/ Ecology
/ Ferns
/ Forestry
/ Hydrogen Peroxide - metabolism
/ Mixed Function Oxygenases - chemistry
/ Mixed Function Oxygenases - genetics
/ Mixed Function Oxygenases - metabolism
/ Plants, Genetically Modified
/ Polysaccharides - metabolism
/ Proteins
/ Receptors, Cell Surface - chemistry
/ Receptors, Cell Surface - genetics
/ Receptors, Cell Surface - metabolism
/ Structure-function relationships
/ Tectaria
