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Manipulation of the microbiota of mass-reared Mediterranean fruit flies Ceratitis capitata (Diptera: Tephritidae) improves sterile male sexual performance
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Manipulation of the microbiota of mass-reared Mediterranean fruit flies Ceratitis capitata (Diptera: Tephritidae) improves sterile male sexual performance
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Journal Article
Manipulation of the microbiota of mass-reared Mediterranean fruit flies Ceratitis capitata (Diptera: Tephritidae) improves sterile male sexual performance
2010
Overview
The sterile insect technique (SIT) is a method of biological control whereby millions of factory reared sterile male insects are released into the field. This technique is commonly used to combat the Mediterranean fruit fly (
Ceratitis capitata
, Diptera: Tephritidae). Sterile medfly males are less competent in attracting and mating with wild females, a property commonly linked to the irradiation process responsible for the sterilization. As bacteria are important partners in the fly's life cycle, we used molecular analytical methods to study the community structure of the gut microbiota in irradiated male medflies. We find that the sterilizing irradiation procedure affects the gut bacterial community structure of the Mediterranean fruit fly. Although the Enterobacteriaceae family remains the dominant bacterial group present in the gut, the levels of
Klebsiella
species decreases significantly in the days after sterilization. In addition, we detected substantial differences in some bacterial species between the mass rearing strain
Vienna 8
and the wild strain. Most notable among these are the increased levels of the potentially pathogenic species
Pseudomonas
in the industrial strain. Testing the hypothesis that regenerating the original microbiota community could result in enhanced competitiveness of the sterile flies, we found that the addition of the bacterial species
Klebsiella oxytoca
to the postirradiation diet enables colonization of these bacteria in the gut while resulting in decreased levels of the
Pseudomonas
sp. Feeding on diets containing bacteria significantly improved sterile male performance in copulatory tests. Further studies will determine the feasibility of bacterial amelioration in SIT operations.
Publisher
Nature Publishing Group UK,Oxford University Press
Subject
/ Animals
/ Bacteria
/ Bacteria - isolation & purification
/ Biomedical and Life Sciences
/ Ceratitis capitata - microbiology
/ Ceratitis capitata - physiology
/ Ceratitis capitata - radiation effects
/ Diet
/ Diptera
/ DNA Fingerprinting - methods
/ Ecology
/ Electrophoresis, Polyacrylamide Gel - methods
/ Fruits
/ Gastrointestinal Tract - microbiology
/ Gastrointestinal Tract - radiation effects
/ Insects
/ Klebsiella oxytoca - growth & development
/ Male
/ Microbial Genetics and Genomics
/ Pest Control, Biological - methods
/ Polymerase Chain Reaction - methods
/ Pseudomonas - growth & development
