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Cloning and characterization of Agp1, the gene encoding the small subunit of ADP-glucose pyrophosphorylase from wheat and its relatives
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Cloning and characterization of Agp1, the gene encoding the small subunit of ADP-glucose pyrophosphorylase from wheat and its relatives
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Cloning and characterization of Agp1, the gene encoding the small subunit of ADP-glucose pyrophosphorylase from wheat and its relatives
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Journal Article
Cloning and characterization of Agp1, the gene encoding the small subunit of ADP-glucose pyrophosphorylase from wheat and its relatives
2017
Overview
ADP-glucose pyrophosphorylase (AGP) consists of two large (AGP-L) and two small (AGP-S) subunits; it regulates the limiting step in the biosynthesis of starch. Here, we isolated the full-length cDNA sequence of the AGP-S gene (designed as
Agp1
) from wheat and whole genomic sequences from relatives of wheat. The coding region of
Agp1
ranged from 5948 bp to 7666 bp and contained 9 exons and 8 introns. The full-length cDNA sequence of
Agp1
encoded a polypeptide containing 473 amino acids without transit peptides. The sequence alignment and phylogenetic tree analyses indicated that the
Agp1
cDNA sequence was highly conserved (99.7%) across the diploid Triticeae species examined in this study. Variations in introns caused the
Agp1
genomic sequences from wheat relatives to be less similar (79.5%), and
Agp1
sequences from the B genome donor species were divergent. In developing grains, rapid increase in
Agp1
expression at prophase was closely followed by the accumulation of starch, and a significant (
P
< 0.001) correlation was observed between them. The heterogeneous mixtures of small and large subunits from different wheat species restarted glycogen synthesis in the
Escherichia coli
AC70R1-504 cells, which are deficient in endogenous AGP activity (
glg C
-/-
). Our data provide useful information to evaluate and utilize
Agp1
sequences in Triticeae species.
Publisher
Springer International Publishing,De Gruyter,Springer Nature B.V
