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D: -Xylose Isomerase from a Marine Bacterium, Vibrio sp. Strain XY-214, and D: -Xylulose Production from β-1,3-Xylan
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D: -Xylose Isomerase from a Marine Bacterium, Vibrio sp. Strain XY-214, and D: -Xylulose Production from β-1,3-Xylan
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Journal Article
D: -Xylose Isomerase from a Marine Bacterium, Vibrio sp. Strain XY-214, and D: -Xylulose Production from β-1,3-Xylan
2012
Overview
The xylA gene from a marine bacterium, Vibrio sp. strain XY-214, encoding D: -xylose isomerase (XylA) was cloned and expressed in Escherichia coli. The xylA gene consisted of 1,320-bp nucleotides encoding a protein of 439 amino acids with a predicted molecular weight of 49,264. XylA was classified into group II xylose isomerases. The native XylA was estimated to be a homotetramer with a molecular mass of 190 kDa. The purified recombinant XylA exhibited maximal activity at 60°C and pH 7.5. Its apparent K m values for D: -xylose and D: -glucose were 7.93 and 187 mM, respectively. Furthermore, we carried out D: -xylulose production from β-1,3-xylan, a major cell wall polysaccharide component of the killer alga Caulerpa taxifolia. The synergistic action of β-1,3-xylanase (TxyA) and β-1,3-xylosidase (XloA) from Vibrio sp. strain XY-214 enabled efficient saccharification of β-1,3-xylan to D: -xylose. D: -Xylose was then converted to D: -xylulose by using XylA from the strain XY-214. The conversion rate of D: -xylose to D: -xylulose by XylA was found to be approximately 40% in the presence of 4 mM sodium tetraborate after 2 h of incubation. These results demonstrated that TxyA, XloA, and XylA from Vibrio sp. strain XY-214 are useful tools for D: -xylulose production from β-1,3-xylan. Because D: -xylulose can be used as a source for ethanol fermentation by yeast Saccharomyces cerevisiae, the present study will provide a basis for ethanol production from β-1,3-xylan.
Publisher
Springer-Verlag
Subject
/ bacteria
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Biomedical and Life Sciences
/ Escherichia coli - metabolism
/ Gene Expression Regulation, Bacterial
/ Gene Expression Regulation, Bacterial - physiology
/ Gene Expression Regulation, Enzymologic
/ genes
/ genetics
/ Marine
/ Vibrio
/ Xylans
/ Xylose
/ yeasts
/ Zoology
