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Host-delivered RNAi: an effective strategy to silence genes in plant parasitic nematodes
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Host-delivered RNAi: an effective strategy to silence genes in plant parasitic nematodes
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Host-delivered RNAi: an effective strategy to silence genes in plant parasitic nematodes
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Journal Article
Host-delivered RNAi: an effective strategy to silence genes in plant parasitic nematodes
2007
Overview
Root-knot nematodes (Meloidogyne spp.) are obligate, sedentary endoparasites that infect many plant species causing large economic losses worldwide. Available nematicides are being banned due to their toxicity or ozone-depleting properties and alternative control strategies are urgently required. We have produced transgenic tobacco (Nicotiana tabacum) plants expressing different dsRNA hairpin structures targeting a root-knot nematode (Meloidogyne javanica) putative transcription factor, MjTis11. We provide evidence that MjTis11 was consistently silenced in nematodes feeding on the roots of transgenic plants. The observed silencing was specific for MjTis11, with other sequence-unrelated genes being unaffected in the nematodes. Those transgenic plants able to induce silencing of MjTis11, also showed the presence of small interfering RNAs. Even though down-regulation of MjTis11 did not result in a lethal phenotype, this study demonstrates the feasibility of silencing root-knot nematode genes by expressing dsRNA in the host plant. Host-delivered RNA interference-triggered (HD-RNAi) silencing of parasite genes provides a novel disease resistance strategy with wide biotechnological applications. The potential of HD-RNAi is not restricted to parasitic nematodes but could be adapted to control other plant-feeding pests.
Publisher
Berlin/Heidelberg : Springer-Verlag,Springer-Verlag,Springer,Springer Nature B.V
Subject
/ Biological and medical sciences
/ Caenorhabditis elegans - classification
/ Caenorhabditis elegans - genetics
/ Caenorhabditis elegans - growth & development
/ Fundamental and applied biological sciences. Psychology
/ Genes
/ genetics
/ Host-Pathogen Interactions - genetics
/ Nematoda
/ Nematoda - growth & development
/ Phytopathology. Animal pests. Plant and forest protection
/ Plant physiology and development
/ Plants
/ Plants, Genetically Modified
/ Proteins
/ Reverse Transcriptase Polymerase Chain Reaction
/ RNA, Double-Stranded - genetics
/ RNA, Double-Stranded - metabolism
/ RNA, Small Interfering - genetics
/ RNA, Small Interfering - metabolism
/ roots
/ tobacco
/ toxicity
