Journal Article

Expression of the FAP gene in non-fibroblast human cell lines. Development of cancer-associated fibroblast models

Alekseenko, I. V., Tyulkina, D. V., Pleshkan, V. V., Sverdlov, E. D., Kopantseva, M. R.

2016

Overview

The fibroblast activation protein (FAP) is selectively expressed in cancer-associated fibroblasts (CAFs) and facilitates tumor progression, which makes this protein an attractive therapeutic target. There are difficulties in obtaining CAFs for studying the function and suppression of FAP. In this work, the expression level of FAP was determined by PCR assay in 25 human cell lines and 8 surgical samples of tumor stroma. The expression of FAP was observed in all tumor stroma samples and in four cell lines: NGP-127, SJCRH30, SJSA-1, and A375. The level of FAP expression in NGP-127, SJCRH30, and SJSA-1 lines as well as in CAFs of patients was comparable, which makes these cell lines a possible model for studying FAP.

• Publisher: Pleiades Publishing,Springer Nature B.V
• Subject: Biochemistry / Biological and Medical Physics / Biomedical and Life Sciences / Biophysics / Blotting, Western / Cancer / Cancer-Associated Fibroblasts - metabolism / Cell Line, Tumor / Cellular biology / Fibroblast activation protein / Fibroblasts / Gelatinases - metabolism / Gene Expression / Humans / Life Sciences / Membrane Proteins - metabolism / Molecular Biology / Neoplasms - metabolism / Neoplasms - surgery / Polymerase Chain Reaction / Protein expression / RNA, Messenger - metabolism / Serine Endopeptidases - metabolism / Stroma / Stromal Cells - metabolism / Therapeutic targets / Tumor cell lines / Tumors