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Chia seed as a source of in vitro establishment of Salvia hispanica l. Plants
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Chia seed as a source of in vitro establishment of Salvia hispanica l. Plants
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Chia seed as a source of in vitro establishment of Salvia hispanica l. Plants
Chia seed as a source of in vitro establishment of Salvia hispanica l. Plants
Journal Article

Chia seed as a source of in vitro establishment of Salvia hispanica l. Plants

2020
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Overview
أجريت هذه الدراسة بهدف إيجاد برنامج متكامل لإكثار نبات الشيا خارج الجسم الحي باستعمال تقانة زراعة الأنسجة النباتية. درِسَ تأثير منظمات النمو النباتية بتراكيز مختلفة في مراحل النشوء، التضاعف و التجذير مع تحديد الظروف الملائمة لإنجاح نقل النباتات النسيجية الى تربة الحقل. أمكن التوصل الى تحفيز التضاعف الخضري و تكوين فروع عدة من أصل قمة نامية بزراعتها في وسط غذائي مجهز بـ 1 ملغم/لتر BAP، إذ بلغت نسبة نشوء الفروع 80% ، معدل عدد الفروع 3.20 فرع/قمة نامية بمعدل طول 3.26 سم. أما عملية التجذير، فقد تمت بزراعة الفروع المكثرة على وسط MS بنصف قوة أملاحه مضافاً له 1 ملغم/لتر IBA و أعطت أعلى نسبة للتجذير و معدل عدد و طول للجذور بلغت 100% ، 5.60 جذر/فرع و 3.40 سم بالتتابع. أشارت نتائج أقلمة النباتات المجذرة الى نسبة بقاء بلغت 85% . Technique of tissue culture for Chia (Salvia hispanica) micropropagation was achieved, this study investigated the impact of various concentrations of plant growth regulators on shoot multiplication and root induction with the Chia's mature seed as a source explant. The highest percentage of shoot formation (80% ), shoots number per explant(3.20) and shoot length(3.26 cm), were recorded on MS medium enriched with BAP(1.0 mgl-1) after eight weeks of seed culture. The optimal medium for the rhizogenesis was achieved on half strength MS medium fortified with 1.0 mgl-1 IBA after four weeks of culture, which had the highest rooting percentage (100% ) with highest mean of roots number (5.6 roots per shoot) with (3.40 cm root length). The rooted plants were successfully adapted ex vitro with a survival rate of 85% .